丹参酮ⅡA诱导人小细胞肺癌细胞凋亡及其分子机制  

Tanshinone ⅡA Induce Human Small Cell Lung Cancer Cells Apoptosis and Molecular Mechanism

作　　者：王江峰[1] 陈晟[1] 

机构地区：[1]浙江省肿瘤医院胸外科,杭州310022

出　　处：《医学研究杂志》2017年第10期84-87,共4页Journal of Medical Research

基　　金：浙江省中药管理局基金资助项目(2015ZA039)

摘　　要：目的了解丹参酮ⅡA对人小细胞肺癌细胞(H446细胞)的凋亡诱导作用,并探讨其可能的分子机制。方法采用MTT法检测不同浓度丹参酮ⅡA对人小细胞肺癌H446细胞增殖的影响结果;Hoechst 33258法检测不同浓度丹参酮ⅡA对人小细胞肺癌H446细胞凋亡的影响;q PCR法检测不同浓度丹参酮ⅡA对人小细胞肺癌H446细胞凋亡基因的影响;Westen blot法检测不同浓度丹参酮ⅡA对人小细胞肺癌H446细胞凋亡的影响。结果不同药物浓度的丹参酮ⅡA(0.3、0.6、1.25、2.5、5和10μg/ml)抑制人小细胞肺癌H446细胞增殖且呈浓度和时间依赖性;随着药物浓度的提高,人小细胞肺癌H446细胞凋亡小体的形成数量增加,高浓度组差异有统计学意义(P<0.05);高浓度组丹参酮ⅡA促进凋亡基因(Bax,caspase-9、caspase-3)的表达,抑制凋亡抑制基因(Akt,Bcl-2)的表达且差异有统计学意义(P<0.05);高浓度组丹参酮ⅡA促进凋亡蛋白(Bax,caspase-9,caspase-3)的表达,抑制凋亡抑制蛋白(Akt,Bcl-2)的表达且差异有统计学意义(P<0.05)。结论丹参酮ⅡA可能是通过Akt-Bax/bcl-2-caspase9-caspase3信号通路诱导人小细胞肺癌H446细胞的凋亡。Objective To know the Tanshinone ⅡA induce human small cell lung cancer cells （H446 cells） apoptosis, and discuss the possible molecular mechanism. Methods Determined by MTT method of different concentrations of Tanshinone ⅡA impact on human small cell lung cancer H446 ceils proliferation; Determined by Hoechst 33258 method of different concentrations Tanshinone ⅡA influence on human small cell lung cancer H446 cells apoptosis ; Determined by qPCR method of different concentrations of Tanshinone ⅡA impact on human small cell lung cancer H446 cells apoptosis gene; Determined by Westen blot method of different concentrations of Tanshinone ⅡA effect on lung cancer H446 cells apoptosis. Results Different drug concentrations of Tanshinone ⅡA （0.3, 0.6, 1.25, 2.5, 5 and 10μg/ml） inhibit human small cell lung cancer H446 cells proliferation and depend by the concentration and time; With the increase of the concentration of the drug in the formation of human small cell lung cancer H446 cells apoptosis corpuscle number increase, signifi- cant difference was found in high concentration group （ P 〈 O. 05） ; High concentration group of Tanshinone I1 A promote apoptosis gene （ Bax, caspase - 9, caspase - 3） expression, and inhibit apoptosis inhibiting genes （ Akt, the Bcl - 2） expression and there were signifi- cant differences （P 〈 O. 05） ; High concentration group of Tanshinone ⅡA promoting apoptosis protein （ Bax, caspase - 9, caspase - 3 ） expression, and inhibit apoptosis inhibiting protein （Akt, the Bcl -2） expression and there were significant differences （P 〈 0.05 ）. Conclusion Tanshinone ⅡA may be through Akt - Bax/Bcl - 2 - caspase - 9 - caspase - 3 signaling pathways induced human small cell lung cancer H446 ceils apoptosis.

关 键 词：丹参酮ⅡA人小细胞肺癌 细胞增殖 凋亡基因 

分 类 号：R73[医药卫生—肿瘤]