重组鸡GSTA3蛋白对福美双诱导肉鸡TD的血清GST-α含量的影响  

Effect of Recombinant Broiler Protein GSTA3 on the Expression of GST-α in Serum of TD Induced by Thiram

作　　者：李欣[1] 李桢[1] 李娜娜[1] 张旭华 张靖 陈柳妃 宁官保[1] 张鼎[1] 田文霞[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《山西农业科学》2017年第11期1853-1855,1858,共4页Journal of Shanxi Agricultural Sciences

基　　金：晋中市重点科技创新平台项目(P171002-3);山西省回国留学人员科研资助项目(2017-073);山西省科技攻关项目(20130311027-3);2017年地方高校国家级大学生创新创业训练计划项目(201710113001);2017年山西省高等学校大学生创新创业训练计划项目(2017081)

摘　　要：为研究重组鸡GSTA3蛋白对肉鸡TD抗氧化功能的影响,先构建肉鸡TD模型,即通过肌肉注射不同剂量(20,50μg/kg)的原核表达鸡GSTA3蛋白,运用ELISA技术检测血清中GST-α的含量。结果表明,与空白组相比,饲喂福美双第1,2,4天,血清GST-α的含量显著上升(P<0.05),注射蛋白组与饲喂福美双组相比,试验第1天,GST-α的含量没有显著差异,试验第2,4天,GST-α的含量显著降低(P<0.05),而高剂量组与低剂量组之间GST-α的含量无显著性差异。GSTA3可通过修复肝脏损伤增强其抗氧化能力,直接或间接调节软骨组织中软骨细胞在TD发生过程中的功能。To study the effect of the recombinant chicken GSTA3 protein on the anti-oxidative function of TD of broiler,the broiler TD model was constructed firstly,and the chicken GSTA3 protein was expressed by intramuscular injection of different doses（20,50 g/kg）,and the content of GST-α in serum was detected by ELISA.The results showed that compared with the control group,the content of GST-α significantly increased in the first and second days（P0.05）.Compared with the feeding and injecting groups,there was no significant difference of GST-α on the first day,but the content of GST-α significantly decreased at 2,4 days（P0.05）,and there was no significant difference between high dose group and low dose group.We suggest that GSTA3 can enhance the antioxidant capacity of the liver by repairing the liver injury,directly or indirectly regulate the cartilage cells in the cartilage cells in the process of TD.

关 键 词：肉鸡 胫骨软骨发育不良 重组鸡谷胱甘肽硫转移酶 抗氧化功能 

分 类 号：S831.1[农业科学—畜牧学]