布拉酵母菌对胃黏膜上皮细胞损伤的保护作用及机制  

Protective effect of Saccharomyces boulardii on injury of human GES-1 cells and the possible mechanisms

作　　者：关月 李超[2] 韩维维[2] 张振玉[2] 

机构地区：[1]南京总医院消化内科,江苏南京210002 [2]南京医科大学附属南京医院消化科,江苏南京210006

出　　处：《中国微生态学杂志》2017年第9期1012-1017,共6页Chinese Journal of Microecology

基　　金：南京市科技发展项目(YKK16135)

摘　　要：目的研究布拉酵母菌对胃黏膜上皮细胞损伤的保护作用。方法建立人胃黏膜上皮细胞GES-1单层细胞模型,并将其分为对照组,布拉酵母菌上清培养组,阿司匹林处理组(18.32mmol/L),阿司匹林+布拉酵母菌上清液组。采用流式细胞术检测各组细胞凋亡率;采用免疫荧光、Western blot方法检测胃黏膜上皮细胞中Occludin和ZO-1蛋白、JNK蛋白、ERK蛋白、磷酸化JNK(p-JNK)蛋白、磷酸化ERK(p-ERK)蛋白表达水平。结果流式细胞术显示阿司匹林+布拉酵母菌上清液组凋亡率低于阿司匹林处理组,差异有统计学意义(P<0.05);免疫荧光结果显示,阿司匹林+布拉酵母菌上清液组Occludin蛋白、ZO-1蛋白表达较阿司匹林处理组增加,Western blot结果显示,阿司匹林+布拉酵母菌上清液组的该两种蛋白表达量较阿司匹林处理组增加,阿司匹林+布拉酵母菌上清液组p-ERK、p-JNK蛋白表达较阿司匹林处理组减少。结论布拉酵母菌对阿司匹林造成的胃黏膜上皮细胞损伤有保护作用,其机制可能通过抑制MAPK信号通路中ERK、JNK的激活而上调Occludin和ZO-1蛋白的表达。Objective To investigate the protective effect of Saccharomyces boulardii on the injury of human GES-1cells induced by Asprin,its possible mechanisms and clinical usage.Methods GES-1cells were cultured in vitro,and divided into four groups：Control group,Saccharomyces boulardii group,Aspirin group,and Aspirin＋Saccharomyces boulardii group.The apoptosis rates of GES-1cells in the four groups were tested with Flow cytometry.The levels of Occludin as well as ZO-1,JNK,ERK,p-JNK and p-ERK were determined by using immunofluorescence and Western blot.Results Flow cytometry showed that the apoptosis rate was 48.30%±1.63%in Asprin group,significantly higher than the 25.83%±2.00%in Aspirin＋Saccharomyces boulardii group（P〈0.05）.Immunofluorescence revealed that the levels of ZO-1and Occludin were increased in Saccharomyces boulardii＋Aspirin group,which was confirmed by Sestern blot.The expression of p-ERK and p-MAPK was lower in Saccharomyces boulardii＋Asprin group than in Aspirin group,according to Western blot.Conclusion Saccharomyces boulardii has a protective effect on the injury of human GES-1cells induced by aspirin,the mechanism of which is probably the inhibition of both ERK and JNK signaling pathways and the up-regulation of the expression of Occludin and ZO-1proteins.

关 键 词：布拉酵母菌 紧密连接 OCCLUDIN ZO-1 MAPK信号通路 

分 类 号：R57[医药卫生—消化系统]