Survivin基因调控Notch1信号通路抑制骨髓瘤细胞增殖及侵袭的机制  

Effects of Survivin gene on the proliferation and invasion of myeloma cells by Notch1 signaling pathway

作　　者：任婧婧 牟佼 李光 张韵洁 刘志勤[2] 戴进前 刘锋[1] 宋艳萍 REN JinNing;MOU Jiao;LI Guang;ZHANG Yunjie;LIU Zhiqin;DAI Jinqian;LIU Feng;SONG Yanping(Institute of Hematology;Department of Neurology, Xi＇an Central Hospital, Xi＇an Jiao Tong University, Xi＇an 710003, Shaanxi, China)

机构地区：[1]西安交通大学附属西安市中心医院血液病研究所,西安7100030 [2]西安交通大学附属西安市中心医院神经内科,西安7100030

出　　处：《癌症进展》2017年第9期1013-1016,共4页Oncology Progress

摘　　要：目的探讨Survivin基因调控Notch1信号通路对骨髓瘤细胞增殖及侵袭的影响。方法 RT-PCR检测42例骨髓瘤组织及正常骨髓组织中Survivin基因的m RNA表达;NC-si RNA和Survivin-si RNA转染人骨髓瘤细胞株RPMI8226,Western blot检测48 h后各组细胞中Survivin蛋白的表达;台盼蓝拒染法和Transwell小室分别检测细胞活率及侵袭能力;Western blot检测MMP-2、MMP-9、Notch1、Hes1蛋白的表达。结果 Survivin基因在骨髓瘤组织中的m RNA表达显著高于正常骨髓组织(P﹤0.01);转染si RNA后RPMI8226细胞中Survivin蛋白的表达显著降低(P﹤0.01);与对照组及NC-si RNA组的比较,Survivin-si RNA组细胞活率、细胞侵袭数及MMP-2、MMP-9、Notch1、Hes1蛋白表达均显著降低(P﹤0.01)。结论 RNA干扰Survivin基因的表达可降低RPMI8226细胞活率及侵袭能力,其机制与抑制Notch1信号通路有关。Objective To investigate the effect of Survivin gene on the proliferation and invasion of myeloma cells by Notch1 signaling pathway. Method The m RNA expression of Survivin gene in 42 cases of bone marrow tumor tissue and normal bone marrow was detected by RT-PCR; NC-si RNA and Survivin-si RNA were transfected to human myeloma cell line RPMI8226, the expression of Survivin protein was detected by Western blot after 48 h; cell activity and invasion ability were detected by trypan blue exclusion and Transwell assay; the expression of MMP-2, MMP-9, Notch1, Hes1 protein were detected by Western blot. Result The m RNA expression of Survivin gene in bone marrow tumor tissues was significantly higher than in normal bone marrow（P0.01）; the expression of Survivin protein after transfection of si RNA in RPMI8226 cells decreased significantly（P0.01）; compared with the control group and NC-si RNA group, the cell activity, cell invasion and MMP-2, MMP-9, Notch1 and Hes1 protein expression in Survivin-si RNA group were significantly reduced（P0.01）. Conclusion The interference of Survivin gene expression by RNA can decrease the proliferation and invasion of RPMI8226 cells, and its mechanism is related to the inhibition of Notch1 signaling pathway.

关 键 词：SURVIVIN基因 骨髓瘤 增殖 侵袭 Notch1信号通路 

分 类 号：R733.3[医药卫生—肿瘤]