miRNA-135在调控前列腺癌细胞增殖中的应用及机制  

Mechanism of miRNA-135 in the regulation of prostate cancer cell proliferation and its application

作　　者：赵宇明 张悦 王华[1] 郭冬梅[1] 田宝[1] 张立民[1] 郑龙[3] ZHAO Yuming;ZHANG Yue;WANG Hua;GUO Dongmei;TIAN Bao;ZHANG Limin;ZHENG Long(Department of Urology, Qinhuangdao First Hospital, Qinhuangdao 066000, Hebei, China;Department of Gynaecology, Qinhuangdao City Maternity and Child Care Hospital, Qinhuangdao 066000, Hebei, China;Laboratory of Molecular Biology, Hebei Medical University, Shijiazhuang 050017, Hebei, China)

机构地区：[1]秦皇岛市第一医院泌尿外科,河北秦皇岛066000 [2]秦皇岛市妇幼保健院妇科,河北秦皇岛066000 [3]河北医科大学分子生物学研究室,石家庄0500170

出　　处：《癌症进展》2017年第9期1017-1019,共3页Oncology Progress

摘　　要：目的观察miRNA-135在调控激素非依赖性前列腺癌细胞增殖能力中的作用及其机制。方法利用微小核糖核酸(miRNA)基因芯片检测激素非依赖性前列腺癌和激素依赖性前列腺癌组织中miRNA-135的表达差异。经过体外转染miRNA-135后对DU145和PC3细胞进行MTT检测,了解细胞的增殖情况。利用miRNA-135靶基因预测软件miRwalk和miRanda预测,初筛miRNA-135调控的基因和相关通路。结果 miRNA基因芯片结果显示,激素依赖性前列腺癌组织中miRNA-135的表达量高于激素非依赖性前列腺癌组织(P﹤0.05);miRNA-135可以抑制DU145和PC3细胞的生长,尤其在第48 h和第72 h以后(P﹤0.05);转染miRNA-135后,前列腺癌细胞株DU145和PC3中STAT6的表达水平较转染NC的细胞株明显下调。结论 miRNA-135能够抑制激素非依赖性前列腺细胞DU145和PC3的增殖能力,有望成为前列腺癌治疗的新靶标。Objective To investigate the role of miRNA-135 in regulating the proliferation of hormone-independent prostate cancer cells and its mechanism. Method The difference in miRNA-135 expression between hormone-independent prostate cancer and hormone-dependent prostate cancer was detected by micro RNA（miRNA） microarray analysis.Transfected DU145 and PC3 cells with miRNA-135 in vitro, then analyzed by MTT to detect cell proliferation. miRNA-135 target–gene–prediction–software miRwalk and miRanda were adopted to preliminarily screen miRNA-135-regulated genes and their related pathways. Result miRNA microarray results showed that the expression of miRNA-135 in hormone-dependent prostate cancer was higher than that in hormone-independent prostate cancer（P0.05）; miRNA-135 could significantly inhibit the growth of DU145 and PC3 cells（P0.05）, especially after 48 h and 72 h. After miRNA-135 transfection, the expression level of STAT6 in prostate cancer cell line DU145 and PC3 was significantly down-regulated compared with NC-transfected cell lines. Conclusion miRNA-135 can inhibit the proliferation of hormone-independent prostatic cell DU145 and PC3, which is expected to be a potential new therapeutic target for prostate cancer.

关 键 词：miRNA-135 前列腺癌 细胞增殖能力 miRNA基因芯片 预测软件 

分 类 号：R737.25[医药卫生—肿瘤]