自组装纳米粒子防龋疫苗的免疫效能  

Study on Immune Efficacy of Self-assembling Anti-caries Nanoparticle Vaccine

作　　者：叶倩琳 李祯 曹茜茜[1] 刘畅[1] 樊明文[1] 

机构地区：[1]口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室武汉大学口腔医学院,湖北武汉430079 [2]武汉大学中南医院麻醉科,湖北武汉430071

出　　处：《口腔医学研究》2017年第10期1023-1026,共4页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:210900019)

摘　　要：目的:研究自组装纳米粒子防龋疫苗Glu-FTH以及Glu+Poly(I:C)(联合应用佐剂Poly(I:C)与抗原Glu)对小鼠特异性体液免疫和黏膜免疫的影响。方法:将小鼠随机分为6组,分别将Glu-FTH、Glu、Glu-FTH+Poly(I:C)、Glu+Poly(I:C)、FTH、PBS经鼻黏膜滴注免疫小鼠,酶联免疫吸附试验(ELISA)检测血清和唾液中特异性抗体水平。结果:Glu-FTH、Glu、Glu-FTH+Poly(I:C)、Glu+Poly(I:C)均可有效提高小鼠血清中抗Glu IgG水平;Glu+Poly(I:C)和Glu可有效提高小鼠唾液中抗Glu sIgA水平。结论:防龋疫苗Glu-FTH具有一定的免疫效果,佐剂Poly(I:C)与抗原Glu联合应用可引发较强的免疫反应。Objective： To study the efficacy of self--assembling anti--caries nanoparticle vaccine Glu--FTH and Glu ＋ Poly （I：C） on inducing protective humoral immune response and mucosal immune response in mice. Meth- otis： The mice were separated into 6 groups randomly. Glu--FTH, Glu, Glu--FTH＋Poly （I：C）, Glu＋Poly （I： C）, FTH and PBS were respectively delivered into mice by intranasal administration. The levels of specific antibod- ies in serum and saliva were tested by enzyme--linked immunosorbent assay （ELISA）. Results： The levels of serum anti--Glu IgG in GIu--FTH, Glu, Glu--FTH＋Poly （I：C）, Glu＋Poly（I：C） group increased. The levels of sali- vary anti-- Glu sIgA in Glu＋ Poly（I： C） and Glu also increased. Conclusion： Anti-- caries vaccine Glu-- FTH and the synergy of Poly （I：C） and Glu can induce specific immune response.

关 键 词：龋齿 自组装纳米粒子疫苗Poly(I:C) 佐剂 黏膜免疫 

分 类 号：R781.1[医药卫生—口腔医学]