16S rRNA/ITS基因序列分析与微生物分类鉴定及其在药品微生物质量控制中的应用  

Application of 16S rRNA/ITS Sequencing and Analysis in the Identification,Classification of Microbial and Drug Quality Control

作　　者：张国林 苏远科[3] 沈海英 顾珉 邢以文 

机构地区：[1]苏州市药品检验检测研究中心,江苏苏州215000 [2]南京中医药大学,南京210000 [3]龙口出入境检验检疫局,山东龙口265700

出　　处：《中国现代应用药学》2017年第10期1489-1495,共7页Chinese Journal of Modern Applied Pharmacy

基　　金：苏州市科技发展计划项目(SYS201425);山东省出入境检验检疫局项目(SK201618)

摘　　要：经典的微生物鉴定有赖于微生物的纯培养,其在一些难培养、生长缓慢或需要特殊营养的微生物鉴定方面有明显的局限性。全自动细菌鉴定及药敏分析系统和基质辅助激光解离飞行时间质谱对微生物鉴定属表型鉴定,严重依赖仪器生产厂商提供的数据库,对类型多样的环境微生物的鉴定分型有明显缺陷。基于遗传物质的分子生物学鉴定技术为微生物的准确鉴定及分型提供了新的思路。16S rRNA/ITS遗传信息具有相对稳定性和易变异的双重特点,不依赖于微生物的营养及生长状态,在微生物的鉴定、分型中得到广泛应用。微生物是影响药品质量的重要因素,而药品微生物控制是药品质量控制的重要方面。本文对16S rRNA/ITS序列与微生物鉴定、分型及其在药品微生物质量控制领域中的应用进行综述,为相关工作提供参考。Traditional methods for identification and classification of microorganisms have limitations in those of unculturable, slow-growth and special dietary needed bacteria. Fully automatic bacteria identification and drug susceptibility analysis system mad MALDI-TOF MS are belonged to phenotype identification which is dependent obviously on the database of equipment. These two methods, are defective i~ ttae variety kinds of environmental microorganisms. Modem molecular based identification methods supply new idea for the environmental microorganism analysis. 16S rRNA/ITS, a sequence contains conservative and variant region is not related with the culture state is widely used in the identification and classification of microorganisms. Microorganism is a signifint factor for drug quality control. The present dissertation tried to explore the structure of 16S rRNA/ITS and its application on drug quality control area.

关 键 词：微生物鉴定 溯源分析 形态学鉴定 16S RRNA 16S-23S rRNA基因间隔区 分子生物学技术 

分 类 号：R915[医药卫生—微生物与生化药学]