八种水果多酚的定量分析与抗氧化活性研究  

Comparative Study on the Phenolic Profiles of Eight Fruits and Their Antioxidant Activities

作　　者：赵谋明[1,2,3] 董红竹 林恋竹[1,2] 

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]广东省食品绿色加工与营养调控工程研究技术中心,广东广州511458 [3]北京工商大学北京食品营养与人类健康高精尖创新中心,北京100048

出　　处：《现代食品科技》2017年第10期225-236,共12页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31501424);广东省科技计划项目(2011A020102001)

摘　　要：本文对比研究了8种水果的果皮、果肉、果汁及全果中维生素C与总酚含量,采用高效液相色谱法测定其多酚种类及含量,并评价其抗氧化活性。8种水果Vc含量从高到低,依次为:番石榴>脐橙>菠萝=木瓜>青苹果=皇冠梨=金桔=芒果;总酚含量最高的水果为芒果;从8种水果中共鉴定出16种多酚类化合物,以绿原酸、儿茶素、表儿茶素、芦丁、杨梅酮和根皮苷含量较高。根据8种水果全果中多酚物质种类与含量,分为4类:(1)脐橙;(2)芒果;(3)金桔;(4)青苹果、皇冠梨、番石榴、菠萝和木瓜;DPPH自由基清除能力从强到弱,依次为芒果>番石榴>青苹果>皇冠梨=金桔=脐橙=菠萝=木瓜;氧自由基吸收能力从强到弱,依次为脐橙>番石榴>皇冠梨=芒果>青苹果=金桔=菠萝=木瓜。本研究可为水果精深加工及功能性食品开发提供理论指导。The content of vitamin C and total phenolic content in peel, flesh, whole fluff and fluit juice of eight fruits were determined in this study. Phenolic profiles of eight fruits were determined by high performance liquid chromatography （HPLC）, and their antioxidant activities were evaluated. The results showed that the content of vitamin C in eight fruits were in the order of guava 〉 navel orange 〉 pineapple = papaya 〉 green apple = crown pear = kumquat = mango. Mango had the highest total phenolic content. Sixteen phenolic compounds were identified fi＇om eight fi＇uits, and the content of chlorogenic acid, catechins, epicatechin, rutin, myricetin and phloridzin were higher. Eight fluits were divided into four categories in terms of the phenolic profiles： （I） navel orange; （2） mango; （3） kumquat; （4） green apple, crown pear, guava, pineapple and papaya. DPPH radical scavenging activities of the eight fruits were in a descending order as follows： mango 〉 guava 〉 green apple 〉 crown pear = kumquat = navel orange = pineapple = papaya. Oxygen radical absorbance capacities decreased in the order of navel orange 〉 guava 〉 crown pear = mango 〉 green apple = kumquat = pineapple = papaya. This study could provide the theoretical guidance for the development of fruit deep processing industry and functional food.

关 键 词：水果 总酚 DPPH自由基清除能力 氧自由基吸收能力 高效液相 

分 类 号：O657.72[理学—分析化学] TS255.1[理学—化学]