车前子降糖活性部位的化学成分研究  

Chemical constituents from hypoglycemic active part of Plantaginis Semen

作　　者：彭庆华 韩竹箴 童仁超 胡信权 杨启明 祁萌[1] 石燕红[1] 王峥涛[1] 杨莉[1,2] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201203 [2]上海中医药大学中医方证与系统生物学研究中心,上海201203

出　　处：《中国中药杂志》2017年第21期4150-4153,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2012ZX09103-201-045);上海市科学技术委员会科技支撑项目(1540192400)

摘　　要：该文研究车前子降糖活性部位的化学成分。前期研究表明车前子60%乙醇提取物对高脂饲料诱导糖尿病模型小鼠的血糖及糖耐量具有一定改善作用。该文进一步应用硅胶、ODS、Sephadex LH-20柱色谱,中压制备液相、薄层制备等色谱技术对车前子的60%乙醇提取物进行化学成分研究,共分离了8个化合物,并根据化合物的理化性质结合应用波谱技术鉴定了化合物结构,分别为车前子萜A(1)、iridolactone(2)、pedicularislacton(3)、rehmaglutin C(4)、京尼平苷酸(5)、p-hydroxylphenylglycerol(6)、4-(2-羟乙基)-1,2-苯二酚(7)、3-buten-2-one-4-[3-(β-D-glucopyranosyloxy)-4-hydroxyphenyl](8)。其中,化合物1~5为环烯醚萜类,6~8为酚酸类。其中化合物1为新的天然产物,化合物2~4,6,8为首次从车前科植物中分离得到。The chemical constituents of Plantaginis Semen with hypoglycemic effect was investigated in this paper. The previous re- suits of the in vivo hypoglycemic effect showed that 60% ethanol extract of Plantaginis Semen decreased the levels of FBG and improved the glucose tolerance in high fat diet（HFD）-induced diabetic C57BL/6 mice. Then, in the present study, the above potential bioactive extract was separated and purified by silica gel, ODS, Sephadex LH-20 column chromatography, medium pressure liquid chromatogra- phy（MPLC） and preparative HPLC. The structures of isolated compounds were identified by physicochemical properties and spectral analyses. Eight compounds were obtained and identified as 4, 4a, 5, 7a-tetrahydro-7-（hydroxymethyl） cyclopenta [ c ] pyran-3 （1 H ）- one（ 1 ）, iridolactone （ 2 ）, pedicularislacton （ 3 ）, rehmaglutin C （ 4 ）, geniposidic acid （ 5 ）, p-hydroxylphenylglycerol （ 6 ）, 1, 2- benzenediol-4- （2-hydroxyethyl） （ 7 ）, and 3-buten-2-one-4- [ 3- （fl-D-glucopyranosyloxy） -4-hydroxyphenyl ] （ 8 ）. Among them, com- pounds 1-5 were iridoids, and 6-8 were phenolic acids. Compound 1 was a new natural product, and compounds 2-4, 6 and 8 were iso- lated from the Plantaginaceae family for the first time.

关 键 词：车前子 化学成分 降糖活性 环烯醚萜 酚酸类 

分 类 号：R284.1[医药卫生—中药学]