Fmr1基因敲除小鼠大脑的磁共振波谱研究  

Brain magnetic resonance spectroscopy of Fmr1 knockout mice

作　　者：郭艺[1] 陈盛强[2] 黄勇[1] 谭理连[1] 彭土康 林秋喜 

机构地区：[1]广州医科大学附属第二医院放射科,广东广州510260 [2]广州医科大学附属第二医院神经研究所,广东广州510260 [3]广州医科大学第二临床学院,广东广州510182

出　　处：《广东医学》2017年第22期3393-3396,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:31771327);广东省科技计划项目(编号:2015A0030302090);广州市医药卫生科技项目(编号:20141A011087;20151A011080)

摘　　要：目的探讨氢质子磁共振波谱成像(~1H-MRS)测定Fmr1基因敲除小鼠(KO小鼠)脑内代谢物的变化及诊断价值。方法 23只4月龄KO小鼠(实验组)和16只野生型FVB小鼠(WT小鼠,对照组)采用3.0T高场强MRI行~1H-MRS,测量小鼠大脑皮质与海马区N-乙酰天门冬氨酸(NAA)、胆碱(Cho)和肌酐(Cr)的峰下面积,计算NAA/Cr和Cho/Cr比值并进行两样本均数t检验。结果与对照组比较,实验组小鼠NAA/Cr比值明显降低(t=-5.912,P<0.01),Cho/Cr比值明显升高(t=3.888,P<0.01)。结论~1H-MRS能定量测定KO小鼠脑内代谢物(细胞膜代谢)异常,可能为诊断及评价脆性X综合征疗效提供重要依据。Objective To investigate the changes of metabolites in brain of Fmrl knockout mice （KO mice） by 1H - MR spectroscopy （ 1H - MRS） and its application in diagnosis of Fragile X Syndrome （ FXS ）. Methods 1H - MRS was performed on 23 KO mice （experimental group） and 16 wild type mice （control group） at the age of 4 months. The sub - peak areas of N - aeetyl aspartate （NAA） , choline （Cho） and creatine （Cr） in the cerebral codex and hippo- campus were measured, and the NAA/Cr and Cho/Cr ratios were calculated and compared by two - sample t - test. Results Compared with control group, the NAA/Cr ratio was significantly reduced （ t = - 5. 912, P 〈 0.0l ） , and the Cho/ Cr ratio was significantly increased （t = 3. 888, P 〈 0. 01 ） in KO mice. Conclusion 1H - MRS examination can quantitatively assess abnormal metabolites in the brains of KO mice, which may provide important clues for the diagnosis of FXS and evaluation of therapeutic efficacy.

关 键 词：脆性X综合征 FMR1基因敲除小鼠 磁共振波谱成像 

分 类 号：R445.2[医药卫生—影像医学与核医学]