SIRT1基因多态性与中国南方汉族人群脑出血的相关性研究  

Correlation between silent information regulator 1 polymorphisms and intracerebral hemorrhagesusceptibility in Han ethnic population of South China

作　　者：薛偕华 苏清平[2] 魏伟 沈菊连 夏敏 林志诚 

机构地区：[1]福建中医药大学附属康复医院神经康复科,福建省康复产业研究院,福州350003 [2]福建中医药大学康复医学院,福州350003

出　　处：《中华神经医学杂志》2017年第11期1091-1097,共7页Chinese Journal of Neuromedicine

基　　金：（1）基金项目：福建省康复产业研究院资助项目（2015Y2001-11）（2）基金项目：福建省科技厅科技平台建设项目（2015Y2001）

摘　　要：目的探讨沉默信息调节因子1（SIRTl）基因多态性与脑出血的相关性。方法纳入福建中医药大学附属康复医院自2013年9月1日至2017年5月30日收治的201例脑出血患者为病例组，同期203例健康体检者为对照组。采用限制性片段长度多态性聚合酶链反应（PCR—RFLP）检测SIRTl基因rs7069102、rs2273773、rs7895833位点多态性并分析其与脑出血及血脂水平的相关性。结果（1）与对照组比较，病例组的rs7895833AA基因型频率分布较高，GG＋AG基因型频率分布较低，差异有统计学意义（P〈0．051；rs7069102CC基因型频率较低，GG＋CG基因型频率较高，但差异无统计学意3L（P〉0．05）。rs2273773基因型、等位基因频率在2组间的分布差异无统计学意义（P〈0．05）。（2）Logistic回归分析显示，rs7895833AA基因型携带者较对照组罹患脑出血风险增]Jn（OR=1．570，95％CI：1．140～2．180，P=0．006）。r31与rs2273773TT基因型比较，CC基因型携带者、CT基因型携带者高密度脂蛋白胆固醇（HDL-C）水平均明显升高，差异有统计学意义（P〈0．05）。rs7069102、rs7895833基因型与血脂水平之间未见联系。结论SIRTl基因rs7895833位点与脑出血易感性显著相关．rs2273773基因型影响中国南方汉族人群血浆HDL-C水平．Objective To investigate the association of silent information regulator 1 （SIRT1） polymorphisms with intracerebral hemorrhage （ICH） susceptibility. Methods From September 1, 2013 to May 30, 2017,Han ethnic 201 ICH patients and 203 controls from South China were enrolled in this study. Genotyping and sequencing ofSIRT1 polymorphisms （rs7069102, rs2273773 and rs7895833） were performed by PCR-restriction fragment length polymorphism （PCR-RFLP）. The correlation of SIRT1 polymorphisms with ICH was analyzed. Results （1） The rs7895833 A allele frequency distribution was significantly higher and the rs7895833 GG＋AG gene frequency distribution was significantly lower in the ICH group than those in the control group （P〈0.05）; the rs7069102 C allele frequency distribution was lower and the GG＋CG gene frequency distribution was higher in the ICH group than those in the control group, without significant differences （P〉0.05）. （2） Logistic regression analysis indicated rs7895833 AA genotype carriers had increased risk for ICH （OR： 1.57, 95%CI：1.14-2.18,P=-0.006）. （3） As compared with patients with rs2273773 TT genotype, patients with CC and CT genotypes had significantly higher high-density lipoprotein cholesterol level （P〈0.05）; there were no associations between rs2273773/rs7069102 and ICH. Conclusion SIRT1 rs7895833 is significantly associated with ICH susceptibility; rs2273773 genotypes affect plasma high-density lipoprotein cholesterol level in the Chinese Han ethnic population.

关 键 词：沉默信息调节因子1基因 脑出血 单核苷酸多态性 

分 类 号：R743.34[医药卫生—神经病学与精神病学]