粪便中Ras相关序列家族2A基因甲基化与大肠癌的关系  

Study on the correlation between Ras related sequence family 2A gene methylation and colorectal cancer in feces

作　　者：孙冬生[1] 魏素菊[3] 王明君[2] 孙敬国[2] 张凤青[1] 

机构地区：[1]唐山市开滦总医院肿瘤科,063000 [2]唐山市开滦总医院普通外科,063000 [3]河北医科大学第四医院肿瘤科,石家庄050011

出　　处：《中华实验外科杂志》2017年第11期1969-1971,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨粪便中的Ras相关序列家族2A（RASSF2A）基因甲基化对大肠癌患者临床诊断的价值。方法使用改良的COBRA法对102例大肠癌粪便中RASSF2A基因启动子两个区域（区域1和区域2）甲基化状态进行了分析。51例结肠镜阴性的健康者为对照组。同时探讨RASSF2A基因甲基化与肿瘤临床病理特征的关系。结果大肠癌患者粪便中广泛甲基化、部分甲基化及无甲基化检出率分别为63%（64/102）、87%（89/102）和13%（13/102），对照组分别为6%（3/51）、22%（11/51）和78%（40/51）。大肠癌患者粪便中广泛甲基化、部分甲基化的检出率明显高于健康体检者，无甲基化检出率明显低于对照组，差异有统计学意义（P＝0.000）。RASSF2基因甲基化与大肠癌患者性别、肿瘤分期、浸润程度之间无显著相关。结论RASSF2A基因甲基化是大肠癌进展过程中的早期事件，粪便RASSF2基因甲基化有望成为早期无创诊断大肠癌的新途径。Objective To investigate the value of methylation of Ras related sequence family 2A （RASSF2A） gene methylation in the stool of patients with eolorectal cancer in the clinical diagnosis of RASSF2A. Methods A modified COBRA method was used to analyze the methylation status of 102 RASSF2A gene promoter regions （region 1 and region 2） in two cases of colorectal cancer. 51 cases of healthy subjects as control group negative colonoscopy. At the same time, the relationship between the methylation of RASSF2A gene and clinical pathological factors. Results In the stools of patients with coloreetal cancer extensive methylation, methylation and methyl of were 87% and 63% （64/102） 89/102 and 13% （13/102） and 6% （3/51） and 22% （11/51） and 78% （40/51） in the control group respec- tively. The detection rate of methylation in stool of colorectal cancer patients was significantly higher than that of healthy persons, and the rate of non methylation was significantly lower than that of the control group, the difference was statistically significant （P = 0. 000）. There was no significant correlation between the methylation of RASSF2 gene and the gender, tumor stage, and the degree of invasion of colorectal cancer patients. Conclusion Methylation of RASSF2A gene is an early event in the progression of colorectal cancer. Methylation of RASSF2 gene in feces is expected to be a new approach to the early diag- nosis of colorectal cancer.

关 键 词：Ras相关序列家族2A基因 甲基化 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]