黑果枸杞花青素生物合成关键基因的生物信息学分析与克隆  

Bioinformatics Analysis and Cloning of the Key Genes Associated with Anthocyanin Biosynthesis fromLyciumruthenicum

作　　者：虎娟 张虹 蒋清安[1,2] 林哲 马伟宝 陈任 

机构地区：[1]宁夏大学西部特色生物资源保护与利用教育部重点实验室 [2]宁夏大学生命科学学院,宁夏银川750021

出　　处：《西北林学院学报》2017年第6期1-11,共11页Journal of Northwest Forestry University

基　　金：2013年度宁夏回族自治区科技支撑计划项目(413-0396)

摘　　要：黑果枸杞(Lycium ruthenicum)是我国西北地区特有的道地药材,是迄今为止发现花青素含量最高的天然野生植物之一。为深入了解黑果枸杞的花青素生物合成机制,利用转录组测序方法,从黑果枸杞果皮中鉴定了参与花青素生物合成的7类关键基因,即查尔酮合酶基因、查尔酮异构酶基因、黄烷酮-3-羟化酶基因、类黄酮-3′-羟化酶基因、类黄酮-3′5′-羟化酶基因、二羟黄酮醇-4-还原酶基因和花青素合酶基因,对其进行相应核苷酸序列、氨基酸序列以及蛋白质(酶)结构特征等生物信息学分析;然后根据转录本拼接序列,利用PCR方法对该7个基因进行了全长克隆和测序验证。为黑果枸杞的遗传改良及花青素基因的利用奠定了分子基础。Lycium ruthenicum is a unique medicinal material in the northwestern China. It contains the high- est content of anthocyanin. To understand more about the biosynthesis mechanism of anthocyanin,in the present research, seven key genes associated with anthocyanin biosynthesis in L. ruthenicum were identified using transcriptome sequencing. The genes including chalconesynthase, chalconeisomerase, clavonone-3- hydroxylase, flavonoid-3＇-hydroxylase, flavonone-3＇5 Chydroxylase, dihydvroflavonol-4-reductase and antho- cyanidin synthase,and their bioinformatics in nucleotide sequences,amino acid sequences and their protein （enzyme） structures were characterized. Then the full lengths of the seven key genes were amplified by PCR according to the transcript assembly sequences, and were verified by sequencing. The research would provide a basis for genetic improvement of L. ruthenicum and the utilization of the genes involved in antho- cyanin biosynthesis.

关 键 词：花青素生物合成 转录组测序 生物信息学分析 PCR克隆 黑果枸杞 

分 类 号：S718.46[农业科学—林学]