口腔扁平苔藓患者外周血单个核细胞和血浆中miR-132的异常表达及其临床意义  

Abnormal expression of miR-132 in peripheral blood mononuclear cells and plasma of the patients with oral lichen planus and its clinical significance

作　　者：陈雪芬 孙晓峰[2] 

机构地区：[1]南京医科大学附属无锡精神卫生中心、南京医科大学附属无锡同仁国际康复医院口腔科,214156 [2]无锡市第二人民医院口腔科

出　　处：《实用口腔医学杂志》2017年第6期812-815,共4页Journal of Practical Stomatology

摘　　要：目的:探讨miR-132在口腔扁平苔藓(OLP)患者外周血单个核细胞(PBMCs)和血浆中的表达及其临床意义。方法:纳入40例OLP患者(24例非糜烂型,16例糜烂型),40名健康对照者;采用实时荧光定量PCR的方法检测2组PBMCs和血浆中miR-132的表达。结果:OLP患者PBMCs和血浆中miR-132的相对表达量均显著高于健康对照组(P<0.01),糜烂型OLP患者PBMC和血浆中miR-132的表达水平均明显高于非糜烂型和健康对照组(P<0.01),而非糜烂型OLP患者PBMCs和血浆中miR-132的表达水平与健康对照组相比,差异均无统计学意义(P>0.05)。结论:miR-132高表达可能与OLP的异常免疫反应有关,并且miR-132可以作为评估OLP病情严重程度的辅助指标。Objective： To investigate the expression of miR-132 in peripheral blood mononuclear cells （PBMCs） and plasma of pa- tients with oral lichen planus （OLP） and the relationship of miR- 132 with clinical forms of OLP. Methods： 40 OLP patients （24 nonerosive OLP, 16 erosive OLP） and 40 healthy controls were included. The relative expression of miR132 in peripheral blood mononuclear cells （PBMCs） and plasma was examined by quantitative real-time PCR. Results： The expression of miR-132 in both PBMCs and plasma of OLP patients was significantly higher than those in healthy controls（ P 〈 0.01 ）. miR- 132 expression of PBMCs and plasma in erosive OLP group was significantly higher than that in non-erosive OLP group and healthy control（ P 〈 0.01 ）. However, the expression of miR-132 in PBMCs and plasma showed no significant difference between non-erosive OLP group and healthy control （P 〉 0.05 ）. Conclusion： miR-132 might be involved in the abnormal immune response of OLP patients, and miR-132 can be utilized as an assistant biomarker for the evaluation of the severity of OLP.

关 键 词：口腔扁平苔藓(OLP) miR-132 外周血单个核细胞 微小 RNA 

分 类 号：R781.5[医药卫生—口腔医学]