胞质M-CSF诱导HeLa细胞侵袭和迁移  

Cytoplasmic M-CSF Induces Invasion and Migration of He La Cells

作　　者：张蒙夏 刘琪[2] 吴海燕 李响[2] 唐圣松 

机构地区：[1]湖南中医药大学组织胚胎学教研室,长沙410208 [2]南华大学组织学与胚胎学教研室,衡阳421001 [3]南华大学药物药理研究所药物蛋白质组学湖南省高等学校重点实验室,衡阳421001 [4]湖南医药学院生物医学研究中心,怀化418000

出　　处：《生物化学与生物物理进展》2017年第11期1007-1015,共9页Progress In Biochemistry and Biophysics

基　　金：湖南省高校创新平台开放基金项目(09K072;12K120);湖南省教育厅青年项目(11B110)资助~~

摘　　要：为探讨巨噬细胞集落刺激因子(M-CSF)对人宫颈癌细胞(He La细胞)侵袭和迁移的影响及机制,采用胞质定位空载体p CMV/cyto/myc与重组载体p CMV/cyto/myc-M-CSF稳定转染He La细胞株,建立稳定高表达胞质M-CSF的细胞系(He La-M细胞).经Transwell实验观察胞质M-CSF对He La细胞侵袭和迁移能力的影响,逆转录-聚合酶链式反应及蛋白质印迹检测细胞Rho三磷酸鸟苷酶(Rho GTPases)及基质金属酶的表达,明胶酶谱法检测基质金属蛋白酶2的活性.结果显示,与转染空载体的He La细胞(He La-C细胞)和对照组He La细胞比较,胞质M-CSF的高表达可明显增强He La细胞在体外的侵袭和迁移能力,其机制与Rho GTPases的活化,以及MMP2表达上调及其活性增高密切相关.In order to study the effects and mechanisms of macrophage colony stimulating factor （M-CSF） on invasion and migration in human cervical cancer cells （HeLa cell line）, the empty vectors （pCMV/cyto/myc） and recombinant vectors （pCMV/cyto/myc-M-CSF） were transfected into HeLa cells to establish the stable cell line with high expression of cytoplasmic M-CSF （HeLa-M cells）. Transwell tests were used to observe the effect of cytoplasmic M-CSF on the cell invasion and migration. MMP-2 activity was detected by gelatin zymography assay. The expression of mRNA and protein was measured by reverse transcription-polymerase chain reaction and Western blotting. The results showed that the high expression of cytoplasmic M-CSF significantly enhanced the ability of invasion and migration ability in HeLa cells, compared with the empty vector transfected HeLa cells （HeLa-C cells） and the control group （HeLa cells）. Our findings suggested that the mechanisms were closely related to the expressions of Rho GTPases （Rac 1, cdc42）, and the increasing activity and expression of MMP2.

关 键 词：巨噬细胞集落刺激因子 宫颈癌 基质金属蛋白酶2 侵袭 迁移 

分 类 号：R737.33[医药卫生—肿瘤]