维生素K2调控Wnt/β-catenin信号对肝癌细胞侵袭、凋亡的影响及机制研究  

The effect and mechanism of vitamin K2 on invasion and apoptosis of hepatocellular carcinoma cells by the regulation of Wnt/β-catenin signaling

作　　者：张锋[1] 苗江永[2] 

机构地区：[1]保定市第二中心医院消化内科,河北保定072750 [2]河北医科大学第二附属医院神经内科

出　　处：《胃肠病学和肝病学杂志》2017年第11期1222-1225,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨维生素K2调控Wnt/β-catenin信号对肝癌细胞侵袭、凋亡的影响及机制。方法 5、10、20、40、80μmol/L的维生素K2处理人肝癌SMMC-7721细胞24、48、72 h后,CCK8实验检测细胞的增殖情况;22μmol/L的维生素K2处理细胞48 h后,Transwell小室检测细胞的侵袭能力;流式细胞仪检测细胞凋亡;Western blotting检测MMP-2、MMP-9、Cleaved caspase3、β-catenin、Cyclin D1蛋白表达。结果 10、20、40、80μmol/L的维生素K2处理肝癌细胞不同时间均可显著抑制癌细胞的增殖,具有浓度依赖性和时间依赖性(P<0.01)。根据IC50值选择22μmol/L的维生素K2为研究对象,维生素K2组细胞的凋亡率及Cleaved caspase3蛋白表达均显著高于对照组,细胞侵袭数及MMP-2、MMP-9、β-catenin、Cyclin D1蛋白表达均显著低于对照组(P<0.01)。结论维生素K2可通过下调Wnt/β-catenin信号抑制肝癌细胞的增殖及侵袭能力,诱导细胞凋亡。Objective To investigate the effect and mechanism of vitamin K2 on invasion and apoptosis of hepatocel- lular carcinoma cells by the regulation of Wnt/β-catenin signaling. Methods 5, 10, 20, 40, 80 p.mol/L vitamin K2 treated human hepatocellular carcinoma cell line SMMC-7721 for 24, 48, 72 hours, CCK8 assay was used to detect cell proliferation ; Transwell assay was performed to detect cell invasion after 22 μmol/L vitamin K2 treated cell for 48 hours ; cell apoptosis was detected by flow cytometry; expressions of MMP-2, MMP-9, Cleaved caspase3, β-catenin and Cyclin D1 protein were detected by Western blotting. Results The proliferation of cancer cells was inhibited after 10, 20, 40, 80 μmol/L vitamin K2 treated cells with different time, with the concentration and time dependence （P 〈 0.01 ）. 22 μmol/L vitamin K2 was selected as the research object according to the IC50 value; cell apoptosis rate and the expression of Cleaved caspase3 protein in vitamin K2 group were significantly higher than those in the control group, the number of cell invasion and expressions of MMP-2, MMP-9, β-catenin, Cyclin D1 protein were significantly lower than those in the control group （P 〈 0.01 ）. Conclusion Vitamin K2 can inhibit the proliferation and invasion of hepatoeel- lular carcinoma cell and induce apoptosis by clown regulating the Wnt/β-catenin signaling.

关 键 词：维生素K2 Wnt/β-catenin信号 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]