5-羟色胺在非糜烂性反流病中表达的研究  

Expression of 5-hydroxytryptamine in non-erosive reflux disease

作　　者：姜志茹[1] 赵雷[1] 郭海[2] 郑爱萍[3] 李明华[4] 

机构地区：[1]北京大学深圳医院老年科,广东深圳518036 [2]北京大学深圳医院消化科,广东深圳518036 [3]北京大学深圳医院病理科,广东深圳518036 [4]北京大学深圳医院中心实验室,广东深圳518036

出　　处：《胃肠病学和肝病学杂志》2017年第11期1282-1284,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：深圳市科技计划项目(JCYJ20150403091443306)

摘　　要：目的研究5-羟色胺(5-hydroxytryptamine,5-HT)在质子泵抑制剂(proton pump inhibtor,PPI)疗效差的非糜烂性反流病(non-erosive reflux disease,NERD)患者和PPI有效的NERD患者食管黏膜中的表达。方法北京大学深圳医院门诊60例NERD患者分为两组:PPI疗效差组21例,PPI有效组39例,10名健康志愿者为正常对照组,胃镜下取食管黏膜标本。免疫组化法测定肥大细胞的表达,免疫印迹法测定5-HT4受体、5-羟色胺转运体(serotonin transporter,SERT)的蛋白表达。结果 PPI疗效差组与PPI有效组的肥大细胞表达分别为:(17.05±2.13)%、(18.37±3.86)%,两组间差异无统计学意义,均高于正常对照组(3.94±2.13)%(P<0.05)。PPI疗效差组、PPI有效组和正常对照组的5-HT4R蛋白吸光度比值分别为:1.08±0.17、0.96±0.15、1.10±0.18,各组间差异无统计学意义(P>0.05)。SERT蛋白吸光度比值:PPI疗效差组(1.22±0.20)>PPI有效组(0.63±0.14)>正常对照组(0.21±0.09)(P<0.05)。结论 SERT的高表达可能参与了PPI疗效差的NERD的发病机制。Objective To investigate the expression of 5-hydroxytryptamine （5-HT） in non-erosive rellux disease （NERD） with poor effection and good effection of proton pump inhibitor （PPI）. Methods Sixty patients of NERD were divided into poor effection of PPI group （21 patients） , good effection of PPI group （39 patients） and 10 volunteers as the normal control group, esophageal mucous membrane biopsy were obtained by gastroseopy. The expression of mast cells was determined by immunohistochemistry technique, the expressions of 5-HT4 receptor and serotonin transporter （SERT） were determined by Western blotting. Results The mast cell expression rate was （17.05 ± 2. 13）% in poor effection of PPI group and （18.37± 3.86）% in good effection of PPI group （with no statistical difference）, higher than that in the normal control group （ 3.94 ± 2.13 ） % （ P 〈 0.05 ）. The 5-HT4 receptor （5-HT4R） protein absorbance ratio by Western blotting were 1.08 ±0.17, 0.96 ±0.15 and 1.10 ±0.18 in poor effection of PPI group, good effection of PPI group and normal control group, respectively, with no statistical difference （P 〉 0.05）. The SERT protein absor- bance ratio： poor effection of PPI group （ 1.22 ±0.20） 〉 good effection of PPI group （0.63 ±0.14） 〉 normal control group （0.21 ± 0.09） （P 〈 0.05）. Conclusion The up-regulation of SERT protein expression might be involved in the pathogenesis of NERD with poor effection of PPI.

关 键 词：5-羟色胺 非糜烂性反流病 质子泵抑制剂 

分 类 号：R57[医药卫生—消化系统]