阿帕替尼对宫颈癌细胞增殖及化疗敏感性的影响  

Effect of apatinib on inhibiting proliferation and increasing chemosensitivity of cervical cancer cells

作　　者：刘秋利 李婧[3] 李娟[4] 齐晓薇[1] 陈玲[1,2] 唐美 余进进 邱海峰[5] 王元[1] Liu Qiuli;Li Jing;Li Juan;et al(Department of Gynecology ,Affiliated Hospi- tal of Jiangnan University,the Fourth People% Hospital of Wuxi City, Wuxi 214000;Wuxi Medical College, Jiangnan University, Wuxi 214000;Department of Oncology, the First Affili- ated Hospital of Zhengzhou Universit, Zhenzhou 450052;Jiangsu University, Zhenjiang 212013)

机构地区：[1]无锡市第四人民医院妇科,无锡214000 [2]江南大学无锡医学院,无锡214000 [3]郑州大学第一附属医院肿瘤科,郑州450052 [4]江苏大学,镇江212013 [5]郑州大学第一附属医院妇科,郑州450052

出　　处：《现代妇产科进展》2017年第11期806-809,共4页Progress in Obstetrics and Gynecology

基　　金：江苏省无锡市科技局项目(No:CSE31N1418);无锡市卫生局项目(No:MS201417;FYKY201401);无锡市医院管理中心科研项目(No:YGZXG1405);国家自然科学基金资助项目(No:81502262);河南省博士后科研项目(No:2014021);河南省医学科技攻关项目(No:201503045)

摘　　要：目的:探讨阿帕替尼(Apatinib)对人宫颈癌细胞的抑制增殖作用以及对宫颈癌化疗药物敏感性的影响。方法:CCK-8实验和克隆形成实验分别检测apatinib对宫颈癌细胞Si Ha和HCC94的增殖抑制作用,流式细胞术检测apatinib对宫颈癌细胞周期的影响,RT-qPCR检测Cyclin D3 mRNA表达,Western blot检测Cyclin D3蛋白表达。结果:Apatinib呈剂量依赖性抑制宫颈癌细胞的增殖;apatinib可诱导宫颈癌细胞发生G_0/G_1期阻滞,显著降低宫颈癌Si Ha和HCC94细胞中Cyclin D3 mRNA及Cyclin D3蛋白的表达水平(P<0.01);apatinib可提高宫颈癌细胞对紫杉醇的化疗敏感性。结论:Apatinib可通过诱导宫颈癌细胞G_0/G_1期阻滞抑制其增殖,且对紫杉醇抑制宫颈癌细胞的生长有增效作用。Objective ： To investigate the effect of inhibitory porliferation, and chemo- sensitivity of apatinib on human cervical cancer cells. Methods： The effect of porliferation, colo- ny forming ability, cell cycle of apatinib on SiHa and HCC94 cervical cancer cell lines were measured by CCK-8 assay, clone formation test and flow cytometry, respectively. The expression of Cyclin D3 mRNA in cells was examined by RT-qPCR. The expression of Cyclin D3 in cells was detected by Western blot. The effect of chemosensitivity of apatinib to several kinds of an- tineoplastic drug for cervical cancer cell lines were detected by CCK-8 assay. Results：Apatinib inhibited cervical cancer cells growth in a dose-dependent manner, and arrested cell cycle at Go/G1 period. Apatinib can significantly reduce the expressions of Cyclin D3 in a dose-depend- ent manner and can increase the sensitivity to adriamycin and paclitaxel of cervical cancer cells in vitro. Conclusions： Apatinib can inhibit the proliferation, arrest cell cycle at G0/G1 period and enhance chemotherapeutic action of paclitaxel against cervical cancer ceils in vitro.

关 键 词：阿帕替尼 宫颈癌 周期 化疗敏感性 

分 类 号：R737.33[医药卫生—肿瘤]