牡丹籽油软胶囊回复突变试验的研究  

Study on Reversion Mutation Test of Peony Seed Oil Soft Capsule

作　　者：张爽[1] 童琳[1] 许勇[1] 李舜[1] 叶红[1] 

机构地区：[1]安徽省医学科学研究院,安徽合肥230061

出　　处：《安徽农业科学》2017年第33期79-80,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31372417)

摘　　要：[目的]采用鼠伤寒沙门氏菌/哺乳动物微粒体酶试验(Ames)对牡丹籽油软胶囊进行安全性毒理学试验。[方法]试验选用鼠伤寒沙门菌TA97、TA98、TA100和TA102菌株,将牡丹籽油软胶囊配制成浓度为5.0、1.0、0.2、0.04 mg/皿4个剂量,和阳性对照组(叠氮钠、2-氨基芴、敌克松)进行比较,观察各菌株的回变菌落数。[结果]牡丹籽油对4个菌株诱发的回变菌落数均未超过对照组2倍,为阴性结果。[结论]牡丹籽油对鼠伤寒沙门氏菌无直接致突变作用。[Objective ]To study the safe toxicology of Peony Seed Oil Soft Capsule by Salmonella typhimurium /Mammalian Microsomal Enzyme Test （Ames）.[Method]To use Peony Seed Oil and adopt Ames test,Salmonella typhimurium TA97, TA98, TA100 and TA102 strains were selected. Sodium azide,2-aminofluorene,dexon was chosen as a mutagen,respectively add 5.0,1.0,0.2,0.04 mg /dish extract,and statisticalling revertant colonies.[Result]The number of revertant colonies was no more than twice of that of spontaneous colonies for each dosage group and there was no dose-response relationship existed.[Conclusion]Peony seed oil had no mutagenicity in this test.

关 键 词：鼠伤寒沙门氏菌/哺乳动物微粒体酶试验 致突变性 诱变 牡丹籽油 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]