氯己定凝胶和氢氧化钙糊剂抑菌作用的对比研究  

In vitro evaluation of the bacteriostatic effects of chlorhexidine gel and calcium hydroxide

作　　者：李韶容 刘颖[2] 杨子 侯本祥[1] 

机构地区：[1]首都医科大学附属北京口腔医院牙体牙髓科,北京100050 [2]首都医科大学附属北京口腔医院研究所,北京100050

出　　处：《牙体牙髓牙周病学杂志》2017年第11期644-648,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(81170952)

摘　　要：目的:研究氯己定凝胶(CHX)、氢氧化钙糊剂(CH)及其混合物(CHX+CH)对粪肠球菌、牙龈卟啉单胞菌、伴放线放线杆菌、微小小单胞菌的抑菌作用。方法:采用琼脂扩散法测量CHX、CH及CHX+CH的抑菌圈;将以上4种细菌悬液分别稀释成1.5×10~8、3×10~8、6×10~8、9×10~8、12×10~8、15×10~8CFU/mL不同浓度,通过直接接触法比较各药物的抑菌效果,并用Kruskal-Wallis检验进行统计分析。结果:抑菌圈从大到小依次为:CHX、CHX+CH、CH(各药物间相比,P<0.05);对粪肠球菌的抑制效果为:CHX+CH>CHX>CH,而对牙龈卟啉单胞菌、伴放线放线杆菌及微小小单胞菌的抑菌效果为:CHX>CH>CHX(各药物间相比,P<0.05)。结论:氯己定凝胶扩散作用最强,氯己定凝胶和氢氧化钙糊剂混合物直接接触抑菌作用最强。AIM： To evaluate the antibacterial activity of chlorhexidine（ CHX） gel and/or calcium hydroxide（ CH） paste against endodontic pathogens. METHODS： Agar diffusion test and direct contact test were used to obtain the inhibition zone and the minimum inhibition concentration（ MIC） of CHX,CH and CHX ＋ CH against 4 endodontic pathogens. Data were statistically analyzed using Kruskal-Wallis test. RESULTS： The diameter of the inhibition zone of the preparations was as following： CHX 〉 CHX ＋ CH〉 CH（ P 〈 0. 05）. CHX ＋ CH showed the strongest inhibition effect for all the pathogens. In the inhibition of enterococcus faecalis the effeciency of the preparations was CHX ＋ CH 〉 CHX 〉 CH（ P〈 0. 05）; in the inhibition of Porphyromonas gingivalis,Actinobacillus actinomycetem comitans and Parvimonasmicra CHX 〉 CH 〉 CHX（ P〈 0. 05）. CONCLUSION： The diffusion ability of CHX gel was the largest while the direct antibacterial activity of CHX ＋ CH was the strongest against the 4 endodontic pathogens.

关 键 词：氯己定凝胶(CHX) 氢氧化钙糊剂(CH) 粪肠球菌 微小小单胞菌 抑菌活性 

分 类 号：R780.2[医药卫生—口腔医学]