盐酸异丙嗪与DNA相互作用的光谱法研究  

Spectroscopic investigation on the interaction of promethazine hydrochloride and DNA

作　　者：许明玲[1] 张晨 林泳佳 屈炳蓉 洪桂坪 胡钰 刘彬[1] 王新[1] 

机构地区：[1]辽宁大学药学院,辽宁沈阳110036

出　　处：《药学研究》2017年第11期630-633,共4页Journal of Pharmaceutical Research

基　　金：国家自然科学基金(No.21402125);辽宁省教育厅科学研究基金(No.L2016008);辽宁大学"大学生创新创业训练计划"(No.X201610140242)

摘　　要：目的研究盐酸异丙嗪(PMT)与DNA的相互作用。方法通过光谱法、热力学理论及黏度法进行研究。结果盐酸异丙嗪与DNA相互作用形成络合物并淬灭其固有荧光,二者相互作用过程中ΔG0<0。二者结合常数随着温度的升高呈现下降趋势,且ΔS0>0,ΔH<0。氯化钠与磷酸二氢钠的加入对盐酸异丙嗪-DNA体系的荧光强度没有影响;随着盐酸异丙嗪浓度的增加,DNA-溴化乙锭体系的荧光强度逐渐减弱。DNA的相对黏度随着盐酸异丙嗪浓度的逐渐增大而显著升高。结论 DNA以静态方式淬灭盐酸异丙嗪固有荧光。二者之间的作用力主要以静电结合为主。盐酸异丙嗪与DNA相互作用模式为嵌插方式,并且盐酸异丙嗪可能以小沟方向插入DNA分子中。Objective To study the interactions between promethazine hydrochloride( PMT) and DNA. Methods Spectroscopic methods,thermodynamic theory and viscometric method were adopted.Results The fluorescence of DNA was quenched remarkably by PMT due to the formation of complex,Gibbs free energy change( ΔG0) was negative.The binding constant of them was decreased with the increase of temperature,and ΔS> 0,ΔH< 0. The fluorescence intensities of PMTDNA had no changes whether with the adding of Na Cl or Na H2 PO4,with the adding of the PMT,the fluorescence intensities of DNA-EB decreased gradually,the viscosity of DNA increased remarkably with the adding of PMT. Conclusion The binding process between DNA and PMT was a spontaneous process,DNA could quench the intrinsic fluorescence of PMT by static quenching.The mainly interaction force was electrostatic interaction and the mode between DNA and PMT was intercalation binding.It was proposed that PMT can intercalate into DNA from the minor groove.

关 键 词：盐酸异丙嗪 DNA 光谱法 相互作用 结合模式 

分 类 号：R914[医药卫生—药物化学]