‘里昂城’铁线莲组培体系的建立  

Establishment of Regeneration System of Clematis ‘Ville de Lyon'

作　　者：魏淑云[1] 陈建华 张荻[1] 申晓辉[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海上房园艺有限公司,上海201114

出　　处：《上海交通大学学报（农业科学版）》2017年第1期22-28,33,共8页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委科技创新行动计划项目(14Z111220004)

摘　　要：以2年生铁线莲园艺品种‘里昂城’带芽茎段为外植体材料,建立了完整的组织培养体系。结果表明,‘里昂城’铁线莲带芽茎段较适宜的灭菌方法是0.1%HgCl2二次灭菌6min和5min,污染率控制为5.56%;初代培养诱导腋芽的最佳培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA,出芽率为93.33%,出芽指数为1.70;MS+1.0mg/L 6-BA+0.1mg/L NAA+1.0mg/L KT是芽增殖的最佳培养基,增殖系数高达4.07,不定芽生长良好;最佳生根培养基为1/2MS+0.3mg/L NAA+3.0mg/L IBA,生根率高达93.33%;生根苗移栽至珍珠岩和草炭土(1∶1,V/V)混合基质中成活率为73.00%。In vitro regeneration protocol from excised stems of Clematis‘Ville de Lyon'via direct method was developed.The results differed significantly among the various experimental treatments,and low contamination(5.56%)and high germination rate(78.89%)were achieved when stem segments were disinfected with 0.10% HgC12twice(6and 5min);The suitable initial culture medium was Murashige and Skoog(MS)medium supplemented with 1.0 mg/L 6-Benzylaminopurine(6-BA)and 0.1 mg/L 1-Naphthaleneacetic acid(NAA),in which the germination rate was 93.33% with a shoot forming index of1.70.Proliferation rate adventitious bud on MS medium complemented with 1.0 mg/L 6-BA,0.1 mg/L NAA and 1.0mg/L Kinetin(KT)was significantly higher(P<0.05)than the other treatments,in which proliferation coefficient was 4.07.Adventitious buds were rooted well on half strength MS medium(1/2MS)with 3.0mg/L Indole-3-butytric acid(IBA)and 0.3mg/L NAA,and the rooting ratio was 93.33%.The survival rate of seedlings was 73.00%in the substrate mixing of perlite and turfy soil as the rate of 1∶1(V/V).

关 键 词：'里昂城’铁线莲 灭菌时间 组织培养 增殖系数 最佳培养基 

分 类 号：S682.19[农业科学—观赏园艺]