鸟苷酸结合蛋白2调控β-葡聚糖诱导的小鼠树突状细胞成熟  

Guanylate-binding protein 2 regulates the maturation of mouse dendritic cells induced by β-glucan

作　　者：张淑雯[1] 冯同保[1] 宁永玲[1] 张晓航[1] 戚春建[1] 

机构地区：[1]南京医科大学附属常州市第二人民医院中心实验室肿瘤研究所,江苏常州213000

出　　处：《细胞与分子免疫学杂志》2017年第9期1153-1159,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81672799);国家自然科学青年基金(31601156)

摘　　要：目的研究鸟苷酸结合蛋白2(GBP2)对树突状细胞(DC)成熟和功能的影响。方法通过基因芯片和实时定量PCR研究β-葡聚糖诱导的DC基因的变化情况,转染GBP2小干扰RNA(si RNA),敲低GBP2的表达,流式细胞术检测DC表面CD11c、主要组织相容性复合体Ⅱ(MHC-Ⅱ)、CD80水平,ELISA检测细胞上清中白细胞介素6(IL-6)、IL-12p70、TNF-α和IL-10水平。T细胞增殖实验检测全葡聚糖颗粒(WGP)刺激的成熟DC在转染GBP2-si RNA后,对卵清蛋白(OVA)特异性CD4+T细胞的促增殖效应。结果β-葡聚糖诱导后,DC的GBP2 m RNA水平显著降低;敲低DC的GBP2水平后,其表面CD11c、主要组织相容性复合体Ⅱ(MHC-Ⅱ)、CD80水平降低,同时,IL-6、IL-12、TNF-α分泌下降;经WGP刺激的成熟DC在转染GBP2-si RNA后,对OVA特异性CD4+T细胞的促增殖效应降低。结论 GBP2能调控β-葡聚糖诱导的DC成熟,进一步抑制T细胞的增殖。Objective To investigate the effect of guanylate-binding protein 2（ GBP2） on β-glucan-induced maturation and immune response of dendritic cel s（ DCs）. Methods RNA-microarray and real time quantitative PCR were used to investigate the change of genes in DCs induced by β-glucan. Thereafter,DCs were transfected with GBP2 si RNA to knock down the expression of GBP2. Flow cytometry was used to detect the surface markers（ CD11 c,MHC-Ⅱ,CD80） on DCs as well as the proliferation of T cells. Cytokines（ IL-6,IL-12 p70,TNF-α,IL-10） were tested by ELISA. Results The expressions of DC surface markers,including CD11 c,MHC-II,CD80,were significantly down-regulated after the cells were transfected with GBP2 si RNA. Moreover,the production of IL-6,IL-12 and TNF-α were also depressed. OVA specific T-cell proliferation decreased when OT-II T cells were co-cultured with GBP2-si RNA transfected-DCs. Conclusion GBP2 can effectively regulate β-glucan-induced maturation of DCs,thus suppressing the proliferation of T cells.

关 键 词：Β-葡聚糖 树突状细胞 鸟苷酸结合蛋白2(GBP2) 

分 类 号：R967[医药卫生—药理学] R392.12[医药卫生—药学]