‘琼引1号’菠萝蜜组培增殖技术优化  

The Optimization of Tissue Culture Conditions for Proliferation of Jackfruit Cultivar 'Qiongyin 1'

作　　者：郭利军[1] 胡福初[1] 罗志文[1] 何凡[1] 邓会栋 陈哲[1] 范鸿雁[1] 

机构地区：[1]海南省农业科学院热带果树研究所农业部海口热带果树科学观测实验站海南省热带果树生物学重点实验室海南省热带果树育种工程技术研究中心,海口571100

出　　处：《分子植物育种》2017年第10期3976-3983,共8页Molecular Plant Breeding

基　　金：农业部物种品种资源保护项目(热带作物)(151721301354052007);海南省属科研院所技术开发专项(KYYS-2016-09);海南省‘琼引1号’菠萝蜜产期调节关键技术集成与示范共同资助

摘　　要：为建立‘琼引1号’菠萝蜜快速增殖的有效方法,本研究以‘琼引1号’菠萝蜜长约0.50~1.00 cm单芽为外植体材料,结合前期调整培养基大量元素用量以及植物生长调节剂浓度等工作,通过逐步优化法,探讨了NH4NO3、Ca(NO3)2、6-BA+NAA不同配比浓度对菠萝蜜增殖效果的影响和PVP(polyvinylpyrrolidone)抑制外植体褐化的效果。结果表明,适宜‘琼引1号’菠萝蜜增殖培养和生长的NH4NO3浓度为412.50 mg/L,Ca(NO3)2浓度为531.00 m/L,6-BA浓度为1.50 mg/L,NAA浓度为0 mg/L,此时的菠萝蜜长势和增殖效果表现最佳,增殖系数达到3.17。研究还发现,PVP浓度达到0.30 g/L时可有效抑制褐化发生。菠萝蜜组培快繁技术的研发将有效加快菠萝蜜新品种的规模化种植及推广,同时还可为下一步的菠萝蜜分子育种提供技术基础。In this study, in order to establish an effective method for tissue culture rapid proliferation of jackfruit cultivar‘Qiongyin 1＇, single buds were used as explant materials, which is about 0.50~1.00 cm in length. And the tasks for adjusting the quantitation of macro-element in culture medium and the concentration of plant growth regulators were also prepared in the early days. We utilized optimization techniques progressively to investigate the effects of tissue culture proliferation on different concentrations of ammonium nitrate（NH_4NO_3）, calcium nitrate（Ca（NO3）2）, 6-benzylaminopurine（6-BA） and 1-naphthaleneacetic acid（NAA）, as well as the browning effects of explants, which polyvinylpyrrolidone（PVP） inhibited. The results showed that the optimal concentration of NH4 NO,Ca（NO_3）2, 6-BA and NAA was 412.50 mg/L, 531.00 mg/L, 1.50 mg/L, and 0 mg/L, respectively. Meanwhile, the growth vigor of jackfruit and the proliferation effects had the best manifestation with a high propagation coefficient of 3.17. The study also found that there could be browning inhibition when the concentration of PVP was up to0.30 g/L. The research and development of tissue culture and rapid propagation techniques would speed up the large-scale planting effectively of new jackfruit species, moreover, it would provide the technical basis for molecular breeding of jackfruit.

关 键 词：菠萝蜜 增殖 NH4NO3 CA(NO3)2 PVP 

分 类 号：S667.8[农业科学—果树学]