水稻滇粳杂保持系具有的籼型遗传成分比率及遗传多样性分析  

Indica Genetic Component Ratios and Genetic Diversity Analysis of Maintainer Line of Dian-type Japonica Hybrid Rice

作　　者：栾一方 李丹丹 姜珍珍[1,2] 柳展 张江丽[1,2] 闫成强 普世皇[2] 金寿林 文建成 谭学林 

机构地区：[1]云南农业大学稻作研究所,昆明650201 [2]云南省杂交粳稻技术研究中心,昆明650201

出　　处：《分子植物育种》2017年第10期4190-4195,共6页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2016YFD0101101)资助

摘　　要：本研究基于可有效鉴别籼粳基因型的插入/缺失(In Del)标记,用筛选出的23个多态性标记对云南农业大学稻作研究所保育的231份滇型杂交粳稻(滇粳杂)保持系材料具有的籼型遗传成分比例及遗传多样性进行了研究。研究发现,供试保持系材料的籼型遗传成份比率较低,都是Fi≤0.25。其中,具有籼型成分比例0<Fi≤0.05、0.05<Fi≤0.1、0.1<Fi≤0.15、0.15<Fi≤0.2、0.2<Fi≤0.25的材料分别占22.94%、33.33%、26.41%、7.36%和3.03%,没有检测到籼型基因型位点的材料仅有6.93%。由此可知,虽然供试的滇粳杂保持系材料的遗传成份以粳稻为主,但多数都具有一定的籼型遗传成份。研究还发现,虽然供试保持系材料来源较广,但是基于In Del标记的Nei基因遗传多样性指数(H)不高,仅为0.143 2,这很可能是由于In Del标记能检测到的等位位点数没有SSR(simple sequence repeat)标记多。另外还发现,不同来源材料的多样性指数差异较大,变异范围为0.104 4~0.177 5。不同来源材料的遗传多样性指数高低的排列顺序为:中国东北>云南农大稻作所>中国华东>国外>云南其他地方>楚雄州农科院>云南省农科院。In this research, we analyzed the Indica genetic component ratios and genetic diversity of 231 maintainer line materials of Dian-type Japonica hybrid rice from Rice Research Institute of Yunnan Agricultural University with 23 insertion/deletion（In Del） polymorphic markers screened. We found that indica genetic component ratios（Fi） of all the materials were low and Fi≤0.25. The percentage of 0Fi≤0.05, 0.05Fi≤0.1, 0.1Fi≤0.15,0.15Fi≤0.2, and 0.2Fi≤0.25 were 22.94%, 33.33%, 26.41%, 7.36%, and 3.03%, respectively. The percentage of materials without indica genotypes site was 6.93%. The results showed that most of the materials had indica genetic components, although genetic backgrounds of all the materials were dominated by Japonica ones. We also found that Nei＇s genetic diversity index（H） among all the materials was only 0.143 2, although the materials were collected from various sources. Such result might be due to lesser allelic number detected by In Del markers than that detected by simple sequence repeat（SSR） markers. We also found that Nei＇s genetic diversity indexes（H） weredifferent among materials from different sources, and them ranged from 0.104 4~0.177 5. Genetic diversity values of the materials from various sources were ranged as Northeast China RRI-YAU East China Overseas other places of YunnanCAASYAAS.

关 键 词：滇型杂交粳稻 保持系 籼型遗传成分 INDEL标记 遗传多样性 

分 类 号：S511[农业科学—作物学]