断节参抑制α-葡萄糖苷酶活性成分研究  

Study on Active Component Inhibiting α-Glucosidase Inhibito from Cynanchum wallichii

作　　者：毛坤军 张莉 周慧云 黄平 

机构地区：[1]江西医学高等专科学校,江西上饶334000

出　　处：《中国现代中药》2017年第11期1541-1544,共4页Modern Chinese Medicine

摘　　要：目的:寻找断节参中具有抑制α-葡萄糖苷酶活性的成分。方法:采用体外抑制α-葡萄糖苷酶活性模型进行追踪,用各种色谱法分离,根据理化性质和谱学数据鉴定结构,筛选出活性较强的单体化合物并进行酶活性抑制动力学研究。结果:断节参乙醇提取物的乙酸乙酯溶性部位具有显著的抑制α-葡萄糖苷酶活性,从中分离出3个化合物,其中断节参苷H和青阳参苷B两个皂苷类化合物具有较强抑制α-葡萄糖苷酶活性,IC50分别为21.90、35.32 mg·L-1,明显高于阳性对照药阿卡波糖(IC50=1 017.41 mg·L-1)。酶活性抑制动力学反应结果表明,两个皂苷对α-葡萄糖苷酶的抑制类型均为非竞争性抑制剂。结论:断节参苷H和青阳参苷B为首次报道对α-葡萄糖苷酶有抑制活性。Objective： To find α-glucosidase inhibitors from Cynanchum wallichii. Methods： The α-glucosidase in- hibitor was isolated and purified by the bioactivity-guided method in vitro, which was used to analyze the inhibitory activity α- gainst α-glucosidase. The inhibitory kinetic of the isolations was also investigated. Results： The ethyl acetate extract of Cynan- chum wallichii showed strong inhibitory activity and three compounds were isolated and identified, only wallicoside H （IC50 = 21.90 mg .L-1） and otophylloside B showed strong inhibitory activity（ IC50 = 35.32 mg. L-1） which was higher than that of acarbose （IC50 = 1 017.41 mg. L-l）. Both of them showed noncompetitive type on α-glucosidase. Conclusion： It was reported that wallicoside H and otophylloside B have α-glucosidase Inhibitors active for the first time.

关 键 词：断节参 Α-葡萄糖苷酶 断节参苷H 青阳参苷B 抑制类型 

分 类 号：R259.871[医药卫生—中西医结合]