益生菌对极低出生体重早产儿生后第14天肠道菌群的影响  

Effects of probiotics on intestinal microflora in very low birth weight infants on the 14th postnatal day

作　　者：黎红平[1] 马丽亚[1] 肖斌[2] 马端[3] 张春丽[1] 朱传瑞[1] 黄艳[1] 肖克林[4] 

机构地区：[1]暨南大学附属深圳市宝安区妇幼保健院新生儿科,518100 [2]暨南大学附属深圳市宝安区妇幼保健院妇幼研究所,518100 [3]复旦大学代谢分子医学教育部重点实验室 [4]暨南大学附属深圳市宝安区妇幼保健院中心实验室,518100

出　　处：《中华新生儿科杂志（中英文）》2017年第4期264-268,共5页Chinese Journal of Neonatology

基　　金：深圳市科技创新委员会资助项目（JCY20140416085544658）;深圳市宝安区科技创新局资助项目（2014064）

摘　　要：目的 探讨补充益生菌对极低出生体重（very low birth weigh,VLBW）早产儿生后第14天肠道菌群的影响,为VLBW早产儿服用益生菌提供理论依据.方法 选择2015年1～ 12月深圳市宝安区妇幼保健院新生儿重症监护室收治的VLBW早产儿,随机分成益生菌组（服用双歧杆菌和植物乳杆菌联合制剂）和安慰剂组,均在生后第1天开奶,开奶当天至校正胎龄36周每天给予益生菌或安慰剂,收集生后第1天和第14天的粪便样本,采用Illumina MiSeq平台双端测序模式对粪便样本细菌DNA进行16S rRNA基因高通量测序及生物信息分析.结果 共纳入21例VLBW早产儿,其中益生菌组9例,安慰剂组12例,两组临床资料对比差异均无统计学意义（P＞0.05）.生后第1天,两组肠道属水平各菌群丰度对比差异无统计学意义（P＞0.05）,多样性对比差异无统计学意义（P=0.94）;与安慰剂组相比,益生菌组生后第14天粪便中双歧杆菌属、乳杆菌属益生菌相对丰度显著增加（P＜0.05）,鞘氨醇单胞菌属显著下降（P＜0.05）,且多样性指数呈增加态势,但差异无统计学意义（P=0.16）.结论 VLBW早产儿补充益生菌可增加肠道益生菌含量,还可能增加菌群多样性.Objective To establish the theoretical basis of the probiotic application among very low birth weight（VLBW） infants,the efficacy of probiotics on the gut microbiota of VLBW infants on the 14th postnatal day was studied.Method The VLBW infants admitted to BaoAn Maternal and Child Care Hospital from January 2015 to December 2015 were randomly assigned into probiotics group and placebo group.From the first feeding to corrected gestational age of 36 weeks,probiotics group was treated with a combination of Bifidobacterium and Lactobacillus while placebo group with placebo.Fecal samples were collected on the 1st and 14th postnatal day.Total bacterial DNA was extracted and sequenced using high-throughput 16S rRNA gene sequencing on MiSeq sequencing platform.Result A total of 21 VLBW infants were enrolled,9 in probiotics group and other 12 placebo group.No significant differences of clinical data existed between the two group （P 〉 0.05）,The abundance and diversity of microflora （P 〉 0.05） on the first day between the two group were similar.Compared with placebo group,the relative abundance of Bifidobacterium and Lactobacillales in stool samples on the 14th day was significantly increased,while the Halomonas was significantly decreased.The relative abundance of the Shannon-index was increased,but without significant difference （P =0.16）.Conclusion Enteral supplementation of probiotics in VLBW infants may increase probiotic bacterium and microflora diversity.

关 键 词：婴儿 极低出生体重 肠道菌群 基因 RRNA 益生菌 

分 类 号：R722.6[医药卫生—儿科]