全自动体外模拟瘤胃发酵系统:19 kPa以下排气压力不影响发酵特征  

Automated Incubation System in Vitro: Venting Pressure Less Than 19 kPa did Not Affect Fermentation Characteristics

作　　者：龙栋磊 文江南 王敏 王荣[1] 张秀敏[3] 毛宏祥[1] 邓近平 谭支良[3] 

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]湖南畜禽安全生产协同创新中心,长沙410128 [3]中国科学院亚热带农业生态研究所,长沙410125 [4]华南农业大学动物科学学院,广州510640

出　　处：《动物营养学报》2017年第11期4171-4179,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31561143009;31472133);国家科技计划项目(2016YFD0500504);湖南省科技计划项目(2015WK3043);中国科学院青年促进会项目

摘　　要：本试验旨在研究不同排气压力对体外模拟瘤胃发酵特征的影响。选用3只体重相近、装有永久性瘤胃瘘管的成年湘东黑山羊作为瘤胃液供体动物,利用全自动体外模拟瘤胃发酵系统,选用6种发酵底物进行48 h体外发酵,研究9、14和19 k Pa 3种排气压力对总产气量、甲烷产量、产气参数、干物质消失率、发酵液p H以及挥发性脂肪酸产量等发酵参数的影响。结果表明:发酵底物对体外模拟瘤胃发酵参数影响显著(P<0.05);排气压力对体外模拟瘤胃发酵参数没有显著影响(P>0.05)。由此可见,排气压力由9 k Pa增至19 k Pa对体外模拟瘤胃发酵特征无影响。The present study w as conducted to determine the effect of different venting pressure on in vitro fermentation characteristics. Rumen fluid w as collected from three adult Xiaongdong black goats w ith similar w eight and permanent rumen fistula,used automated incubation system in vitro,and six w idely varied substrates w ere selected for 48 h in vitro fermentation,and three venting pressures（ 9,14 and 19 k Pa） w ere performed to investigate the effects of venting pressure on in vitro total gas production,methane production,in vitro dry matter disappearance,fermentation fluid p H and volatile fatty acid production et al. The results show ed that fermentation substrate had significant effects on in vitro rumen fermentation parameters（ P〈0.05）;how ever,venting pressure had no significant effects on in vitro fermentation parameters（ P〉0.05）. In conclusion,venting pressure increase from 9 k Pa to 19 k Pa do not affect fermentation characteristics.

关 键 词：排气压力 体外发酵 气体生成 挥发性脂肪酸 

分 类 号：S816.6[农业科学—饲料科学]