檀香紫檀苗木炭疽病病原菌分离鉴定  

Pathogen isolation and identification of a leaf disease of red sandalwood(Pterocarpus santalinus) seedlings

作　　者：张少平[1] 徐呈祥[1] 李超群 郑福庆[1] 

机构地区：[1]肇庆学院生命科学学院,广东肇庆526061

出　　处：《广东农业科学》2017年第8期85-89,174,共6页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31270674);肇庆学院创新强校重点研究平台(CQ201607)

摘　　要：在檀香紫檀引种繁育中,发现其温室中的苗木冬春时节发生一种较严重的叶部病害,但病害名称、病原菌种类未见报道。以感染该病害的18月龄苗木为试材,分离纯化后获得病原菌株,采用形态学与分子生物学相结合的方法对其进行鉴定。根据分离到的该菌株菌落形态特征、分生孢子形态特征及病原菌r DNA-ITS序列测试分析结果,确定分离到的菌株为胶孢炭疽菌(Colletotrichum gloeosporioides);致病性试验结果表明,胶孢炭疽菌是导致檀香紫檀叶片该种病害的病原菌,但属非典型炭疽病。讨论了檀香紫檀苗木炭疽病的防控。In the introduction and propagation of red sandalwood(Pterocarpus santalinus),we found a serious leaf disease of its seedlings in winter and spring seasons,but the name of the disease and its pathogen species have not been reported.The pathogen isolated from infected leaves of 18-month-old seedlings was identified as Colletotrichum gloeosporioides by morphological characteristics of colony and conidium,and analysis results of r DNA-internal transcribed spacer sequence(ITS) of the strain.Pathogenicity test results further confirmed that C.gloeosporioides was the pathogen responsible for the infected leaves symptoms of red sandalwood,however,the disease belongs to an atypical anthracnose.Control of the leaf diseases of red sandalwood seedlings was discussed.

关 键 词：檀香紫檀 叶部病害 病原菌 分离鉴定 胶孢炭疽菌 

分 类 号：S763.15[农业科学—森林保护学]