水貂冠状病毒中国分离株全基因序列测定及分析  

Whole Genome Sequencing and Analysis on Mink Coronavirus Isolated from China

作　　者：王楷宬[1] 庄青叶[1] 邱源[1] 侯广宇[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国动物检疫》2017年第12期88-91,共4页China Animal Health Inspection

基　　金：国家重点研发计划项目(2017YFC1200500);中国动物卫生与流行病学中心创新基金(2015IF-0004FF)

摘　　要：为分析水貂冠状病毒中国分离株的遗传特征,采用高通量测序方法测定从山东省青岛市某养殖场分离的水貂冠状病毒基因组,首次报道了水貂冠状病毒中国分离株的全基因序列,并对其进行特性分析与比较基因组研究。结果显示:该病毒全基因组大小为28924 bp,具有与其他冠状病毒相似的基因组结构和基因顺序;5′端具有帽子结构,3′端具有poly(A)尾。该分离株与19株代表性毒株的全基因组和N基因进化和同源性分析都证实,该分离株与美国分离株的同源性较高,与其余2株水貂冠状病毒和2株雪貂冠状病毒同属于冠状病毒的一个新种。To analyze the genetic characteristics of Mink coronavirus isolated from China,the genome of Mink coronavirus isolated from a farm of Qingdao city was determined by high throughput sequencing. The full-length genome sequence of the Mink coronavirus Chinese isolate was reported for the ？rst time. The genomic sequence of the isolate was analyzed and compared with reference coronavirus. The results showed that the full-length of the isolate was 28 924 bp,which possessed genomic structure and gene order similar to the reference coronaviruses. The 5＇ end had a hat structure,and the 3＇ end had poly（A）tail. The genome and N gene evolution and homology analysis between the isolate and other 19 representative strains showed that the isolate had a high homology with the American isolate,and was a new species belonging to the coronavirus with the other 2 Mink coronaviruses and 2 ferret coronaviruses.

关 键 词：水貂冠状病毒 中国分离株 基因组分析 高通量测序 

分 类 号：S851.3[农业科学—预防兽医学]