拮抗核盘菌Bt菌株的筛选及抑菌活性研究  

Screening of and studying on Bt strains with antagonistic activity against Sclerotinia sclerotiorum

作　　者：王美玲 耿丽丽[2] 段江燕[1] 张杰[2] 

机构地区：[1]山西师范大学生命科学学院,临汾041004 [2]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护》2017年第6期62-67,共6页Plant Protection

基　　金：国家重点研发计划(2017YFD0201204)

摘　　要：利用平板对峙试验从170株Bt菌株中筛选获得12株对核盘菌Sclerotinia sclerotiorum有抑菌活性的菌株,对其中4株活性较强的菌株进行抗病相关基因分析,发现菌株中都含有几丁质酶基因,测定4AP1菌株发酵液的几丁质酶活力为672.4U/mL。通过对4AP1菌株pH和温度敏感性的研究发现,4AP1菌株最高可耐受55℃高温及pH 12缓冲液的处理,抑菌活性与对照无显著差异。且该菌株抑菌谱较广,除核盘菌外,对小麦赤霉病菌Fusarium graminearum等其他9种病原真菌均有抑制作用。本研究获得的4AP1菌株对核盘菌具有较强抑制作用,且耐热耐强碱环境,为构建杀虫抗病Bt工程菌提供了菌株资源。A total of 12 Bt strains with antifungal activity against Sclerotinia sclerotiorum were obtained from170 strains of Bt strains by the plate confrontation test.Disease resistance-related genes of 4 strains with higher activity were analyzed and all these strains contained chit gene.Chitinase activity of 4 AP1 strain cell culture was 672.4 U/mL.The 4 AP1 strain could withstand high temperature of 55℃ and pH12,and its antifungal activity was not significantly different from the control.Moreover,the antimicrobial spectrum of 4 AP1 strain was broad,except for S.sclerotiorum,which had antifungal activity against the other 9 fungal diseases such as Fusarium graminearum,etc.In this study,4 AP1 strains with strong antifungal activity against S.sclerotiorum,and heat and alkali resistance were obtained,and this provides strain resources for construction of engineering Bt with insecticidal activity and disease-resistance.

关 键 词：核盘菌 BT菌株 抑菌活性 几丁质酶 

分 类 号：S476[农业科学—农业昆虫与害虫防治]