基于富集微流控芯片的稻曲病菌孢子光电检测方法  

Photoelectric detection method for false smut fungal spore based on enrichment microfluidic chip

作　　者：杨宁[1,2] 王盼[1] 张荣标[1] 徐佩锋[1] 孙俊[1] 毛罕平[2] 

机构地区：[1]江苏大学电气信息工程学院,镇江212013 [2]江苏大学农业工程研究院,镇江212013

出　　处：《农业工程学报》2017年第20期161-168,共8页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金(61673195;31701324);江苏省高校优势学科建设工程资助项目(PAPD)(苏政办发[2011]6号);中国博士后特别资助项目(2015T80512);江苏省自然科学基金项目(BK20140550)

摘　　要：针对当前水稻真菌病害发病时间短、传播速度快,缺乏有效早期预警技术的难点,提出一种基于微流控芯片的空气流中水稻真菌病害光电检测方法。该文根据微尺度下孢子富集动力学特征设计了病害孢子高效富集微流控芯片,并结合光电检测系统进行病害孢子的检测。试验根据空气动力学原理以及孢子富集量的大小对微流控芯片通道尺寸进行设置。根据光检测原理和不同浓度孢子在富集区形成的光衰减特性,筛选具有高灵敏度特性的检测光强。在以检测光谱的灵敏度性能为目标并综合考虑线性度的基础上,优选检测波长。以水稻稻曲病菌孢子为检测对象,进行了自动化微流控富集和光电检测试验。试验结果表明:在光源光强1.1×104 cd,波长为650 nm时,利用所述检测方法针对水稻稻曲病菌孢子检测结果(相比与镜检值)误差小于17.5%,根据检测结果建立相关系数为0.992 9的检测模型,具有较好的线性度及可靠性。研究结果为便携式作物病害检测装备的研发提供理论基础。The fungal disease of false smut is research focus, which can spread around within short time and cause much loss of rice yield. However, there hasn't been any effective detection and prediction method. For this reason, we designed a set of sample interface, injection channel, enrichment detection area, electric pumping interface in one of the microfluidic chips. The sample interface is connected to the hose for the collection of fungal spores. The injection channel adopts the simple and gentle straight-through design with a fixed width, in order to achieve the barrier-free movement of the fungal spores in the channel, making it easy to clean the chip, and thus improving the recycle rate of the chip. The enrichment detection area is designed as a circular chamber. This design not only slows the drop rate of the spores, which therefore makes the spores arranged in the enrichment detection area efficiently, but it also matches the circular parallel light spot formed by the fiber output lens. In this way, we can reduce the interference and noise introduced by positioning the light source inaccurately and too large spot diameter. Electric suction interface accesses micro-pump pipeline, and compared to the inflatable design, the pumping design reduces the loss in the pump during the collection of spores. In order to make the fungal spores be arranged in the enrichment detection area correctly, the sample channel width was set in the experiment and the diameter of the enrichment detection area was optimized to meet the needs of fungal spore enrichment speed. The experimental results showed that the enriched detection zone had the best enrichment effect when the diameter was 2 000 μm. Then, a photoelectric detection system based on microfluidic chip was established in this paper. When the spore concentration of microfluidic chip was detected by photoelectric detection method, the system condition parameters mainly included light intensity and wavelength. Therefore, different concentrations of spores were tested by the

关 键 词：病害 灵敏度分析 模型 微流控芯片 真菌 光电检测 

分 类 号：S124.3[农业科学—农业基础科学]