芜青中谷氨酸脱羧酶(Gad)酶性质的时空差异  

The spatial and temporal variation of glutamate decarboxylase(Gad) property in Brassica rapa L.ssp rapa

作　　者：彭彤[1] 郭艳芳 何韵云 郭亦然 唐琳[1] 陈放 PENG Tong;GUO Yan-Fang;HE Yun-Yun;GUO Yi-Ran;TANG Lin;CHEN Fang(College of Life Sciences, Sichuan University, Chengdu 610064, China;School of biological science and medical engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China)

机构地区：[1]四川大学生命科学学院,成都610064 [2]北京航空航天大学生物科学与医学工程学院,北京100191

出　　处：《四川大学学报（自然科学版）》2017年第6期1323-1328,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31100257)

摘　　要：该文对芜青中谷氨酸脱羧酶(Gad)粗酶的提取和优化、酶活力最适反应条件、时空分布差异及光照影响等进行对比分析,同时利用超高效液相色谱法技术(UPLC)分析测定反应产物γ-氨基丁酸(Gaba)的含量.实验结果表明,提取Gad粗酶时加入纤维素酶能提高约70%酶活力.在最适条件对比实验中,对芜青芽苗、块根、茎生长点、茎及顶端分生组织分别进行测定,其中芽苗与顶端分生组织中的酶活力较高.光照对萌发中芽苗的Gad酶活力有较大影响,避光条件下酶活力下降约75%.结果表明,芜青芽苗Gad酶活力好且易于获取,转化谷氨酸反应生成γ-氨基丁酸的转化率高.In this study, crude enzyme extraction method, diversity property and light influence of glutamate decarboxylase（Gad） in Brassica rapa L. ssp rapa were analyzed. Ultra Performance Liquid Chromatography（UPLC） method was used to measure the production in Gad catalyzed progress-Gamma-aminobutyric acid（Gaba）. Adding 1% cellulase solution at homogenization step could improve the enzyme activity up to about 70% as much. Meanwhile, when pH were 4.5-6 with the temperature of 55℃ ,enzyme had reached its maximum activity , on the other hand Ca^2＋ and coenzyme Plp didn＇t have any contribution. Among the different developing stages of Brassica rapa L. ssp rapa, extration of sucking seedling apical meristem could generate more Gaba. In general, with proper conditions, the sucking seedling could be the most efficient material and easily obtained.

关 键 词：芜青 谷氨酸脱羧酶(Gad) 酶活力 γ-氨基丁酸(Gaba) 

分 类 号：Q949[生物学—植物学]