MicroRNA 34a-5p对成骨细胞炎症反应及分化的影响  

Effects of MicroRNA 34a-5p on Inflammation and Differentiation of Osteoblast

作　　者：王贵玲[1] 杨谛[1] 郭佳杰[1] 于雅琼[1] 仇丽鸿[1] 

机构地区：[1]中国医科大学口腔医学院牙体牙髓病科,辽宁省口腔疾病重点实验室,辽宁沈阳110002

出　　处：《口腔医学研究》2017年第11期1139-1142,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81500843);沈阳市科学技术项目计划(编号:F15-199-1-56)

摘　　要：目的:探究microRNA 34a-5p(miR-34a-5p)对成骨细胞炎症反应及分化的调节作用,并初步探究其相关机制。方法:脂质体法瞬时转染人成骨样细胞系MG63使细胞中miR-34a-5p表达增强,同时设通用阴性对照(NC)组。牙髓卟啉单胞菌(Porphyromonas endodontalis,P.e)来源的不同浓度脂多糖(lipopolysaccharide,LPS)作用于MG63后,检测细胞内miR-34a-5p表达;取20mg/L P.e-LPS作用24h后,检测细胞内IL-6表达。成骨诱导48h后,检测细胞中成骨分化标志物以及Notch1受体的表达。结果:P.e-LPS作用于MG63细胞后,miR-34a-5p表达被抑制(P<0.05)。MiR-34a-5p转染细胞后,与NC组相比,细胞内IL-6表达减少(P<0.01);20mg/L P.e-LPS作用下,miR-34a-5p组IL-6表达较NC组下降更为明显(P<0.01)。成骨细胞诱导分化48h后,miR-34a-5p组中分化标志物表达较NC对照组均显著增加(P<0.05),而Notch1受体表达被抑制(P<0.05)。结论:MiR-34a-5p可能通过Notch1信号通路,发挥抑制成骨细胞炎症和促进成骨分化的作用。Objective：To investigate the effect of microRNA 34 a-5 p（miR-34 a-5 p）on the inflammation and differentiation of osteoblasts.Methods：MG63 was transfected with miR-34 a-5 p mimics to ensure a higher expression than general negative control（NC）.Expression of miR-34 a-5 p was detected after MG63 was stimulated by different concentrations of lipopolysaccharide（LPS）from porphyromonas endodontalis（P.e）.Transfected cells were stimulated by 20 mg/L P.e-LPS for 24 h,and expression of IL-6 was detected.Expressions of osteogenic differentiation markers and Notch1 were detected after osteogenic induction for 48 h.Results：Expression of miR-34 a-5 p in MG63 was down-regulated by P.e-LPS with different concentrations（P0.05）.MiR-34 a-5 p inhibited the expression of IL-6 significantly both with and without the stimulation of P.e-LPS（P0.01）.Osteogenic makers in MG63 were promoted by miR-34 a-5 p（P 0.05）and Notch1 was down-regulated（P0.05）.Conclusion：MiR-34 a-5 p inhibits the inflammation and promotes the differentiation of osteoblast,and Notch1 may serve as a target during the process.

关 键 词：MICRORNA 34a-5p 成骨细胞 炎症 分化 

分 类 号：R781[医药卫生—口腔医学]