实验性牙周炎模型大鼠牙槽骨微结构的改变  

Changes of Microarchitecture of Alveolar Bone in Experimental Periodontitis in Rats

作　　者：陈景宜 张昀 陈彩云 程岚[2] 张修银[3] 

机构地区：[1]兰州市口腔医院牙周黏膜科,甘肃兰州730000 [2]上海交通大学医学院附属第九人民医院牙周病科,上海200011 [3]上海交通大学医学院附属第九人民医院口腔修复科,上海市口腔医学重点实验室,上海200011

出　　处：《口腔医学研究》2017年第11期1165-1168,共4页Journal of Oral Science Research

基　　金：上海高校高峰高原学科建设项目(编号:20152523);兰州口腔临床医学中心(2014)

摘　　要：目的:研究牙周炎对大鼠根分叉区牙槽骨微结构的影响。方法:3月龄健康未孕大鼠14只,采用丝线结扎大鼠左侧上颌第一磨牙建立实验性牙周炎模型(实验组),右侧作为对照组。分别于建模后4周及8周时处死大鼠,并测量上颌磨牙牙周探诊深度、收集上颌骨标本。采用Micro-CT分析大鼠上颌第一磨牙根中1/3区牙槽骨微结构的改变。结果:4周及8周实验组牙周探针深度均高于对照组(P<0.05).Micro-CT图像分析发现对照组牙槽骨致密,主要由板状骨小梁构成;实验组牙槽骨变疏松,杆状骨小梁较多。牙槽骨微结构参数分析发现4周及8周实验组骨密度(BMD)、骨体积分数(BV/TV)及骨小梁宽度(Tb.Th.)均较对照组降低(P<0.05),且8周实验组BMD、BV/TV、Tb.Th.较4周组降低(P<0.05)。8周实验组骨小梁数目(Tb.N.)及骨髓腔宽度(Tb.Sp.)较对照组及4周实验组均增加(<0.05)。结论:牙周炎可导致大鼠根分叉区牙槽骨丢失、骨小梁微结构破坏,且这种破坏随着时间延长不断加剧。Objective：To study the changes of the microarchitecture of alveolar bone in the experimental periodontitis in rats.Methods：Fourteen 3-month-old Sprague-Dawley rats were used to establish periodontitis model by ligaturing the left maxillary 1 st molar（PER）,and the right maxillary 1 st molar was used as control（CON）.The rats were sacrificed respectively 4 weeks and 8 weeks later.The maxilla was analyzed by Micro-CT scanning.All statistical analysis was performed with SPSS.Results：The probing depth in PER group increased in comparison to CON group at both 4 and 8 weeks.The alveolar bone of CON group was compacted with plate-like trabecular,while the alveolar bone of PER group become porotic with rod-like trabecular.When compared with corresponding CON group,BMD,BV/TV,and Tb.Th.of alveolar bone significantly decreased in PER group at both 4 and 8 weeks.BMD,BV/TV,and Tb.Th.in 8-week PER group decreased compared with 4-week PER group.Tb.N and Tb.Sp.in 8-week PER group increased in compared with both CON group and 4-week PER group.Conclusion：Periodontitis could induce bone loss and deterioration of trabeculae of alveolar bone.The deterioration might be aggravated by the extended duration.

关 键 词：牙周炎 牙槽骨 MICRO-CT 骨微结构 

分 类 号：R-332[医药卫生] R781.42