温度对海捕野生大黄鱼精子活力的影响  

Effects of Temperature on Sperm Motility of Wild Large Yellow Croaker Pseudosciaena crocea

作　　者：罗芬[1] 黄伟卿[1,2] 阮少江 李进寿[1] 陈小小[1] 阮俊峰 周逢芳[1] 

机构地区：[1]宁德师范学院生物系,福建宁德352100 [2]宁德市鼎诚水产有限公司,福建宁德352100

出　　处：《水产科学》2017年第6期814-817,共4页Fisheries Science

基　　金：福建省科技计划项目(2017N0027);宁德师范学院创新团队建设项目(2015T10)

摘　　要：给7尾体质量325~520g的成熟海捕野生大黄鱼人工催产后采精液,在20℃(室温)和4℃(冰箱)下保存0、1、3、6、12、24、36h,以砂滤海水为激活剂,观察大黄鱼精子活力,以精子的激烈运动、摇尾运动、缓慢运动和寿命作为评价指标。试验结果表明,4℃和20℃下随保存时间的延长,精子活力逐渐减弱;4℃下保存24h、20℃下保存12h,精子仍具有激烈运动的能力;4℃下保存的3个运动阶段和寿命较20℃下均有所延长,4℃保存大黄鱼精子更佳。The activation and motility of spermatozoa collected from captured wild male large yellow croak- er （Pseudosciaena crocea） with body weight of 325--520 g were investigated under the different preserva- tion time （0, 1, 3, 6, 12, 24 h and 36 h） at the temperature of 4 ℃ and 20℃. The strenuous movement, wagging movement, slow movement and life time of sperm were taken as an index to evaluate sperm activi- ty. The results showed that the sperm had gradually weak motility over time at the temperature of 4 ℃ and 20 ℃, being still strenuous movement when the sperm was preserved at 4 ℃ for 24 h or 20 ℃ for 12 h. However, there were longer movement in the three stages of life at 4℃ than at 20 ℃, with stronger motility at 4℃ than at 20℃.

关 键 词：野生 大黄鱼 精子活力 温度 

分 类 号：S965.322[农业科学—水产养殖]