inv(9)血液病患者造血干细胞移植后骨髓造血恢复特征研究  

Study of characteristics of bone marrow hematopoietic recovery after accepting hematopoietic stem cell transplantation in patients with inv(9) blood system diseases

作　　者：张慧[1] 顾国浩[1] 吴丽丽[1] 潘金兰[2] 

机构地区：[1]苏州大学附属第一医院检验科,江苏苏州215006 [2]江苏省血液研究所/卫生部血栓与止血重点实验室,江苏苏州215006

出　　处：《国际检验医学杂志》2017年第22期3128-3130,3133,共4页International Journal of Laboratory Medicine

摘　　要：目的探讨9号染色体倒位[inv(9)]患者在接受造血干细胞移植后中性粒细胞计数(ANC)和血小板计数(PLT)等骨髓造血恢复特征。方法选取2010年1月至2015年10月本院确诊的39 589例血液病患者作为研究对象,采用R显带技术、聚合酶链反应(PCR)技术和流式细胞仪检测技术进行染色体核型检查、融合基因检测和骨髓造血恢复相关指标检测。结果 inv(9)血液病患者检出PML-RARα、BCR-ABL1、AML-ETO、EVI1、CBFβ-MYH11、MLL-AF6、AML-AF4、SET-NUM214、SILTALI、IgH重排、TCR重排和BCL1-IgH等多种融合基因。inv(9)患者在接受造血干细胞移植后恢复情况:ANC在移植后12d恢复至大于0.5×10~9/L水平,PLT在移植后16d恢复至大于20×10~9/L水平。非inv(9)患者在接受造血干细胞移植后恢复情况:ANC在移植后12d恢复至大于0.5×10~9/L水平,PLT在移植后13d恢复至大于20×10~9/L水平。结论 inv(9)血液病患者和非inv(9)血液病患者造血干细胞移植后ANC恢复至大于0.5×10~9/L水平所需的时间几乎相等,而inv(9)血液病患者PLT恢复所需的时间比非inv(9)血液病患者所需时间稍长。Objective To study the characteristics of bone marrow hematopoietic recovery such as absolute neutrophil count（ANC）and platelet（PLT）after accepting hematopoietic stem cell transplantation in the patients with inv（9）.Methods A total of39 589 cases of definitely diagnosed hematonosis in our hospital from January 2010 to October 2015 served as the research subjects.The R banding technique,polymerase chain reaction（PCR）and flow cytometry instrument were adopted to check chromosome karyotype,fusion gene and bone marrow hematopoietic recovery related indicators.Results PML-RARα,BCR-ABL1,AML-ETO,EVI1,CBFβ-MYH11,MLL-AF6,AML-AF4,SET-NUM214,SIL-TALI,IgH rearrangement,TCR rearrangement and BCL1-IgH and other fusion gene were detected in the patients with inv（9）hematonosis.The recovery situation after receiving hematopoietic stem cell transplantation in the patients with inv（9）：ANC recovered to0.5×10~9/L on 12 dafter transplantation,PLT recovered to20×10~9/L on 16 dafter transplantation.The recovery situation after receiving hematopoietic stem cell transplantation in the patients with non-inv（9）：ANC recovered to0.5×10~9/L on 12 dafter transplantation,and PLT recovered to20×10~9/L on 13 d after transplantation.Conclusion The time achieving ANC recovery 0.5×10~9/L after hematopoietic stem cell transplantation in the patients with inv（9）and without inv（9）is almost similar,while the time achieving PLT count recovery in the patients with inv（9）is slightly longer than that in the patients without inv（9）.

关 键 词：9号染色体倒位 血液系统疾病 融合基因 造血干细胞移植 

分 类 号：R457.7[医药卫生—治疗学] R55[医药卫生—临床医学]