ERRα对前列腺癌细胞能量代谢转化的影响  

Effect of ERRα on energy metabolism and transformation of prostate cancer cells

作　　者：郭吉楠[1] 房杰群[1] 袁也晴 江洪涛[1] 邹畅[2] 赵盼 肖克峰[1] 

机构地区：[1]暨南大学第二临床医学院,深圳市泌尿外科微创工程中心,深圳市人民医院泌尿外科,广东深圳518000 [2]暨南大学第二临床医学院,深圳市人民医院,临床医学研究中心,广东深圳518000

出　　处：《广州医科大学学报》2017年第3期1-4,共4页Academic Journal of Guangzhou Medical University

基　　金：国家自然科学基金(81402370);广东省自然科学基金(2015A030313829);广东省科技项目产学研合作项目(2015B090904007);广东省科技厅粤港创新圈项目(2016A050503009);深圳市卫生计生科研项目(201402007)

摘　　要：目的:探讨孤儿受体ERRα对前列腺癌细胞能量代谢转化的影响。方法:通过慢病毒介导的FLAG构建ERRα表达稳定上调的LNCa P前列腺癌细胞模型,Western Blotting法检测其表达量,检测常氧和低氧条件下培养的LNCa P和LNCa P-ERRα细胞相关酶活性(乳酸脱氢酶、己糖激酶、丙酮酸激酶)及ADP含量。结果:过表达ERRα的LNCa P细胞的乳酸脱氢酶、己糖激酶、丙酮酸激酶(低氧)活性和ADP的量均明显高于LNCa P细胞(P<0.05)。结论:在前列腺癌细胞中上调ERRα的表达水平能促进其能量代谢转化。Objective：To investigate the effect of orphan receptor ERRα on energy metabolism and transformation of prostate cancer cells. Methods：LNCaP prostate cancer cell model with stable up-regulated expression of ERRα was established by lentivirus-mediated FLAG. The expression level was determined by Western blotting. Cultured under normoxia and hypoxia conditions,the LNCaP and LNCaP-ERRαcells-associated enzyme activities （lactate dehydrogenase,hexokinase and pyruvate kinase） and ADP contents were measured. Results：The activities of lactate dehydrogenase,hexokinase and pyruvate kinase （ hypoxia） and ADP contents in ERRα over-expressing LNCaP cells were significantly higher than those in LNCaP cells （ P〈0.05） . Conclusion：Up-regulating the expression level of ERRα in prostate cancer cells can promote its energy metabolism and transformation.

关 键 词：雌激素相关受体α 前列腺癌 能量代谢 

分 类 号：R737.25[医药卫生—肿瘤]