腺病毒CD-80基因转导B16-F10细胞的体外生物学特征(英文)  

Biological characteristics of adenovirus-CD80-transduced B16-F10 cells in vitro

作　　者：田长富[1] 李殿俊[1] 刘旭[1] 任丽君[1] TIAN Chang-fu LI Dian-jun LIU Xu REN Li-jun 

机构地区：[1]哈尔滨医科大学肿瘤研究所,黑龙江哈尔滨,150040 [2]Cancer Research Institute Harbin Medical Untversity Harbin,150040,China

出　　处：《哈尔滨医科大学学报》2002年第4期263-266,F003,共5页Journal of Harbin Medical University

基　　金：SupportedbytheNationalProgramforKeySciencesandTechnologyProjects,the 9thFiveYearPlan( 96 -90 6 -10 -2 0 ) ;HeilongjiangProv inceNaturalSciencesFoundation(D972 3)

摘　　要：目的　探讨含hB7 1基因的重组腺病毒hCD80rAd对B16 F10肿瘤细胞体外生物学特性的影响。方法　以腺病毒为载体 ,将CD80基因导入B16 F10肿瘤细胞后 ,以PCR方法检测基因导入 ,以电镜、FACS等方法观察对其体外生物学特性的影响。结果　重组腺病毒的滴度可达 10 1 0 PFU mL ,2 0MOIsrAd可使 95 %以上的B16 F10细胞被感染。转染CD80基因后 ,B16 F10细胞的生长能力、克隆形成能力等均无变化 ,电镜下 ,细胞表面结构及胞内超微结构有轻微变化。结论　重组腺病毒hCD80rAd可以有效地将hCD80基因导入肿瘤细胞而不会引起明显的生物学特性改变 。Objective To study the in vitro biological characteristics of hCD80rAd (human CD80 recombinant adenovirus) infected B16-F10 cells on the basis of generation of replication-deficient CD80(hB7) recombinant adenovirus. Methods Human CD80 gene was transduced into B16-F10 cells mediated by recombinant adenovirus and then was detected by PCR and agarose gel electrophoresis .The biological characteristics of the above cells were analyzed with electron microscope, FACS and other methods.Results The titers of rAd reached 10 10 PFU/ml and more than 95% B16-F10 cells could be infected by 20 MOIs rAd. The growth curve and cloning efficiency of hCD80rAd-infected B16-F10 cells showed no significant differences compared with that of the control cells. The cell proliferation cycle of hCD80rAd-infected B16-F10 cells showed no change through FACS test. The surface structure and ultrastructure of B16-F10 cells had a little change after infected by hCD80rAd.Conclusion The hCD80rAd can transfect B16-F10 cells efficiently without changing their biological characteristics significantly.These results will lay the foundation for preparation of tumor vaccines.

关 键 词：体外生物学特征 重组腺病毒 B16-F10 CD80 基因治疗 肿瘤疫苗 

分 类 号：R730.51[医药卫生—肿瘤]