系统性红斑狼疮患儿外周血IL-6、IL-10、IL-17水平变化及其在单个核细胞中的表达研究  

The expression of IL-6, IL-10 and IL-17 in peripheral blood and their mRNA in peripheral blood mononuclear cells from children with systemic lupus erythematosus

作　　者：沈茹[1] 李艳君[2] 林丽佳[3] 

机构地区：[1]昆明市儿童医院检验科,云南昆明650228 [2]昆明市疾病预防控制中心,云南昆明650011 [3]昆明市儿童医院科教科,云南昆明650228

出　　处：《分子诊断与治疗杂志》2017年第6期371-375,共5页Journal of Molecular Diagnostics and Therapy

摘　　要：目的研究系统性红斑狼疮(systemic lupus erythematosus,SLE)患儿外周血中白介素-6(interleukin-6,IL-6)、白介素-10(IL-10)、白介素-17(IL-17)水平的变化情况及其在外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中的表达情况。方法选取46例SLE患儿及20例健康儿童分别为病例组(SLE组)和对照组(Control组),采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测外周血中IL-6、IL-10、IL-17蛋白水平,采用实时荧光定量PCR(quantitative real time polymerse chain reaction,q PCR)技术检测PBMCs中IL-6、IL-10、IL-17的m RNA表达水平,分析比较2组的蛋白和m RNA表达量的差异。结果 SLE组IL-6、IL-10、IL-17蛋白及m RNA表达水平均高于Control组,差异均具有统计学意义(P<0.05)。结论 SLE患儿外周血中IL-6、IL-10、IL-17蛋白及m RNA表达水平升高,提示其可能参与SLE患儿的发病过程。Objective To investigate the interleukin-6（IL-6）, interleukin-10（IL-10） and interleukin-17（IL-17） in peripheral blood from children with systemic lupus erythematosus（SLE）, and detect the expression of IL-6, IL-10 and IL-17 m RNA in their peripheral blood mononuclear cells（PBMCs）. Methods The levels of IL-6, IL-10 and IL-17 were detected by enzyme-linked immunosorbent assay（ELISA） in 46 children with SLE（SLE group） and 20 healthy children（Control group）, and the m RNA levels in PBMCs were detected by quantitative real time polymerse chain reacton（q PCR）. Results The protein and m RNA levels of IL-6,IL-10 and IL-17 in SLE group were significantly higher than those in Control group（P〈0.05）. Conclusion The levels of IL-6, IL-10 and IL-17 proteins and m RNA in peripheral blood of children with SLE increased,suggesting that IL-6, IL-10 and IL-17 may be involved in the pathogenesis of SLE in children.

关 键 词：系统性红斑狼疮 白介素-6 白介素-10 白介素-17 外周血 

分 类 号：R725.9[医药卫生—儿科]