乌梅果超氧化物歧化酶的纯化和部分性质研究  

Superoxide dismutase from fruit of Prunus mume: purification and some properties

作　　者：张尔贤[1] 陈杰[1] 顾伟文 肖湘[1] 俞丽君[1] 吴丹奇[1] 

机构地区：[1]汕头大学生物学系

出　　处：《中国药学杂志》1991年第7期404-406,共3页Chinese Pharmaceutical Journal

摘　　要：采用硫酸铵沉淀和Sephadex G-100、DEAE-DE52分离工艺纯化乌梅（青竹梅）超氧化物歧化酶。SDS凝胶电泳测得其亚基分子量为12600。经凝胶电泳鉴定为单一带。该酶最大紫外吸收为192nm。对2mmol/L KCN和1.5%H2O2表现敏感。Superoxide dismutase (EC. 1.15. 1.1) fromfruit of Prunus mume (Sieb.) Sieb. et Zuce waspurifide with technics of the ammonium sulphateprecipitation and separation of sephadex G100 andDEAE-DE52. Its molecular weight was 12000as estimated with SDS PAGE. The purified en-zyme showed a single band on disc gel electro-phoresis with a maximal ultraviolet absorption rateof 192nm. The purified enzyme was sensitive to 2mmol KCN and 1.5% H_2O_2.

关 键 词：乌梅 超氧物歧化酶 纯化 

分 类 号：R284.2[医药卫生—中药学]