载体介导无遗传物质整合的诱导性多能干细胞的生成  

Generation of Induced Pluripotent Stem Cells without Integration of Genetic Materials Mediated with Vector

作　　者：栗楠[1] 郭红梅 王明玉 令文慧 邱小燕[1] 李跃民[1] 刘旭蕾 邓雅楠[1] 肖雄[1] 

机构地区：[1]西南大学动物科技学院,重庆400715

出　　处：《中国细胞生物学学报》2017年第11期1486-1496,共11页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31572488);重庆市基础科学与前沿技术研究项目(批准号:cstc2017jcyj AX0477);国家级大学生创新创业训练计划项目(批准号:201510635015);中央高校基本科研业务费项目(批准号:XDJK2014C022);西南大学博士基金(批准号:SWU113002)资助的课题~~

摘　　要：诱导性多能干细胞(induced pluripotent stem cells,i PSCs)因其具有类似胚胎干细胞(embryonic stem cells,ESCs)的"自我更新"和分化潜能而成为生物学和医学等领域的研究热点之一。但是,目前i PSCs的生成主要以逆转录病毒或慢病毒为载体,而病毒载体和外源性重编程因子在受体细胞基因组中的整合,使得i PSCs存在致瘤性的安全隐患。同时,采用无载体介导的方法如重组蛋白、小分子物质的重编程效率较低。为此,目前已成功开发一些载体和方法可以介导无外源性遗传物质整合的i PSCs的生成。该文就整合后可删除的基因表达载体(Cre/lox P重组系统、piggy Bac转座子/转座酶系统)和非整合基因表达载体(ori P/EBNA1附加型载体、微环DNA载体)在i PSCs生成上的应用研究作一综述,以期为i PSCs的临床安全应用提供理论和技术参考。Induced pluripotent stem cells （iPSCs） have become one of the research focuses in medicine, biology, and so on, because they have the characteristics of ＂self-renewal＂ and differentiation which are similar to embryonic stem cells （ESCs）. But the generation of iPSCs mainly depended on the expression of transcription factors mediated with retrovirus or lentivirus-based vector, these iPSCs bear certain risks, such as tumorigenicity, which resulted from the integration of exogenous genetic materials and viral vector into genome of recipient cells. At the same time, lower efficiency of reprogramming mediated with substance, such as recombinant proteins or small molecules was observed. Therefore, some vectors and methods were successfully developed to generate iPSCs without insertional transgene expression. This paper reviewed the researches about the applications of geneexpression vector systems including Cre/loxP recombination system, piggyBac transposition system, oriP/EBNA1 episomal vector system and minicircle DNA vector system on the generation of iPSCs to provide a theoretical and technological reference for safely clinical applications ofiPSCs.

关 键 词：诱导性多能干细胞(iPSCs) CRE/LOXP重组系统 piggyBac转座系统 oriP/EBNA1附加型载体 微环DNA载体 

分 类 号：Q813[生物学—生物工程]