铝锰复合胁迫下臭牡丹抗逆性及其DNA损伤的研究  

Research of the Stress Resistance and DNA Damage of Clerodendrum bungei Steud. under Single and Combined Treatment of Al and Mn

作　　者：张建新[1] 闵佳丽 俞沁媛[2,3] 郑佳梦 陈楚 吴玉环 楼泱泱[2,3] 刘鹏 

机构地区：[1]丽水职业技术学院,浙江丽水323000 [2]浙江师范大学生态学研究所,浙江金华321004 [3]浙江师范大学植物学实验室,浙江金华321004 [4]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《生态环境学报》2017年第11期1856-1864,共9页Ecology and Environmental Sciences

基　　金：国家自然科学基金项目(41571049;30540056);浙江省公益技术应用研究计划项目(2015C32127);丽水市重点科技创新团队(2012cxtd08)

摘　　要：以臭牡丹(Clerodendrum bungei Steud.)幼苗为试验对象,配制Al^(3+)浓度梯度为0.00、0.05、0.20、0.40和0.80 mmol·L^(-1),Mn^(2+)浓度梯度为0.00、2.00、4.00、8.00和12.00 mmol·L^(-1)的胁迫液,在土培条件下进行单一和复合胁迫处理,研究10、20、30 d后臭牡丹幼苗的抗氧化酶含量等生理应答及DNA状态,以探究臭牡丹对Al、Mn胁迫的抗性,以期为臭牡丹的扩大生产提供参考,为植物抗逆性研究奠定基础。实验结果表明:(1)Al-Mn复合胁迫对3种抗氧化酶的互作效应有所不同,超氧化物歧化酶活性随胁迫浓度升高而降低,最高抑制率达45.1%,趋势与单一胁迫处理大致相似;过氧化物酶活性在复合胁迫下呈先升后降趋势,且低浓度复合胁迫处理所受影响小于单一胁迫处理,高浓度下则抑制效应加重;相较铝、锰单一胁迫处理,低浓度复合胁迫使过氧化氢酶活性分别提高84.1%和140.1%,高浓度则分别降低61.5%和40.1%;(2)Al^(3+)、Mn^(2+)共存时均促进丙二醛、脯氨酸含量的积累,表现为协同效应;(3)利用CASP软件对彗星电泳图像进行分析,结果显示,低浓度Al^(3+)、Mn^(2+)单一及复合胁迫下,臭牡丹根系DNA几乎没有受到损伤,而当胁迫较强时(Al 0.80 mmol·L^(-1)、Mn 12.00 mmol·L^(-1)、Al 0.40 mmol·L^(-1)-Mn 8.00 mmol·L^(-1)、Al 0.80 mmol·L^(-1)-Mn 12.00 mmol·L^(-1)),OTM大幅增加,Al 0.40 mmol·L^(-1)-Mn 8.00 mmol·L^(-1)复合胁迫分别是单一胁迫的1.40倍和1.44倍,且T3较T4增幅达67.9%,说明高浓度胁迫造成的损害较严重,复合胁迫产生的损伤较单一胁迫更强。综合分析认为:低浓度单一金属离子对臭牡丹生长具有一定促进作用,而当铝、锰浓度分别大于或等于0.40 mmol·L^(-1)和8.00 mmol·L^(-1)时则会对其造成损伤,且复合处理对不同指标具有不同效应。因而,在实际大规模生产中应考察土壤金属离子的种类和浓度,依据臭牡丹的耐受程度选择合适的培育土壤。The effects of Aluminum （Al） and Manganese （Mn） on the physiological response and root system DNA damage ofClerodendrum bungei Steud. were tested. We examined the physiological response and DNA damage of Clerodendrum bungeiseedlings till the 10th, 20th and 30th day, after they grew on a variety of soil cultural mediums with different Al3＋ and Mn2＋concentrations, and compared them among different treatments. A range of Al3＋ concentration include 0.00, 0.05, 0.20, 0.40 and 0.80mmol·L-1, while those of Mn2＋ concentration include 0.00, 2.00, 4.00, 8.00 and 12.00 mmol·L-1. Combination of Al3＋ and Mn2＋ haddifferent effects on three antioxidant enzymes. The activity of SOD increased with the concentration decreasing. The highest rate ofinhibition was 45.14%, parallel to those growing on mediums with either Al3＋ or Mn2＋. As for POD, its activity was less affected atrelatively low concentration of combination of Al3＋ and Mn2＋, but more greatly at high concentration, comparing to those growing onmedium with single component. The CAT’s activity at medium with combination of low concentrations of Al3＋ and Mn2＋ was84.10% of those on mediums with only Al3＋, and 140.11% with only Mn2＋. However, at high concentration, these values were downto 61.50% and 61.50% for AL3＋ and Mn2＋, respectively. The coexistence of Al3＋ and Mn2＋ promoted the accumulation of MDA andPro, suggesting the synergy. We investigated the comet electrophore images, and found that the DNA root system was hardlydamaged at low concentrations of neither Al3＋, nor Mn2＋, nor combination. However, the rapid accumulation of OTM at relativelyhigh concentration of either components, such as Al 0.80 mmol·L-1, Mn 12.00 mmol·L-1, Al 0.40 mmol·L-1 -Mn 8.00 mmol·L-1 and Al0.80 mmol·L-1 -Mn 12.00 mmol·L-1, suggesting the existing of DNA damage. Furthermore, these damages were higher at mediumswith combination of components than those at medium with single component. This study explored evidence to support that lowconc

关 键 词：Al MN 复合胁迫 臭牡丹 抗逆性 DNA损伤 

分 类 号：Q945.78[生物学—植物学] X173[环境科学与工程—环境科学]