29种毒力基因在91株食源性单核细胞增生李斯特氏菌中的分布  

Distribution characterization of 29 virulence genes in 91 foodborne Listeria monocytogenes strains

作　　者：李秀娟[1] 赵冬[1] 潘琢[1] 高伟利[1] 徐保红[1] 秦丽云[1] 

机构地区：[1]石家庄市疾病预防控制中心,石家庄050011

出　　处：《中国人兽共患病学报》2017年第11期972-978,共7页Chinese Journal of Zoonoses

基　　金：河北省医学科学研究重点课题(No.ZL20140244)~~

摘　　要：目的了解河北省食源性单核细胞增生李斯特菌(Listeria monocytogenes,Lm)毒力基因分布特征。方法采用普通PCR方法对Lm的29个毒力基因(包括毒力岛Ⅰ在内的6个位点:prfA、plcA、plcB、hlyA、mpl和actA;内化素家族的10个位点:inlA、inlB、inlC、inlD、inlE、inlF、inlG、inlH/C2、inlI和inlJ;其他13个毒力相关位点:bsh、srtA、iap、sigB、virR、mprF、dltA、dltB、dltC、dltD、srtB、fbpA和hpt)进行检测。结果在91株Lm中,有23个毒力基因的检出率为100%。26株Lm的29种毒力基因全部检出,65株Lm存在inlD、inlF、inlG、inlH/C2、inlJ和mpl等6个毒力基因的不同缺失。缺失最严重的为inlG和inlF,缺失率分别为60.44%和54.95%;其次为mpl基因,缺失率为19.78%。根据毒力基因缺失情况,91株菌可分为10个基因型,优势毒力基因型为具有全部23种毒力基因的Ⅰ型。石家庄地区的毒力菌株缺失率(41/52)高于河北北部地区(11/22)。结论河北省食源性Lm的毒力基因携带率高,毒力基因缺失具有多样性且存在地域差异。In order to study the distribution of virulence genes of Listeria monocytogenes(Lm)in Hebei Province,29 virulence genes of Lm,including Listeria monocytogenes pathogenicity islands Ⅰ(LIPI-Ⅰ:prfA,plcA,plcB,hlyA,mpl and actA),10 internalins genes(inlA,inlB,inlC,inlD,inlE,inlF,inlG,inlH/C2,inlIand inlJ)and the other 13 virulenceassociated genes(bsh,srtA,iap,sigB,virR,mprF,dltA,dltB,dltC,dltD,srtB,fbpAand hpt)were detected by PCR.Results showed that in the 91 Lm strains,the detection rate of 23 virulence genes were 100%.The 29 virulence genes of 26 Lm strains were all detected,and 65 Lmstrains had different deletion of 6 virulence genes inlD,inlF,inlG,inlH/C2,inlJand mpl.The deletion rate of inlGand inlF were 60.44% and 54.95%,respectively,following by mpl gene,with a deletion rate of 19.78%.According to the absence of virulence genes,91 strains could be divided into 10 subtypes,and the dominant virulence subtypes was typeⅠwith all 23 virulence genes.The deletion rate of virulent genes in Shijiazhuang was higher than that in northern Hebei.It is suggested that the rate of virulence gene of food-borne Lmin Hebei Province is high,and the virulence gene deletion patterns has diversity and regional differences.

关 键 词：单核细胞增生李斯特菌 毒力基因 分布特征 

分 类 号：R378.99[医药卫生—病原生物学]