鳕鱼皮寡肽对人胃癌细胞SGC-7901增殖凋亡的影响及机制  

Effect of cod skin oligopeptide on the proliferation and apoptosis in human gastric cancer cells line SGC-7901 and its mechanism

作　　者：吴丽萍[1] 续力云 胡晓斐 张国强[1] 

机构地区：[1]浙江大学舟山医院胃肠外科,浙江舟山316021 [2]浙江大学舟山医院分子生物学实验室,浙江舟山316021

出　　处：《解剖学报》2017年第6期698-703,共6页Acta Anatomica Sinica

基　　金：浙江省自然科学基金(LY12C20002);浙江省科技厅公益类项目(2011C23034)

摘　　要：目的探讨鳕鱼皮寡肽(CSO)对人胃癌细胞(SGC-7901)的增殖影响。方法用不同浓度CSO处理体外培养的SGC-7901细胞后,荧光显微镜下观察细胞4'6-二脒基-2-苯基吲哚(DAPI)染色后细胞形态变化,应用CCK-8实验检测细胞活力,免疫印迹法和流式细胞术检测细胞凋亡及细胞周期。结果 CSO对SGC-7901细胞增殖有明显的抑制作用,且并呈剂量、时间效应关系(P<0.05)。作用于SGC-7901胃癌细胞24 h、48 h和72 h的IC50分别是156.90 g/L、102.10 g/L、73.13 g/L。DAPI染色后发现,SGC-7901细胞随着CSO浓度增加可见大量的细胞核碎裂,荧光强烈。24 h细胞凋亡率检测显示,细胞随着浓度的增加凋亡率呈上升趋势,表明CSO对SGC-7901呈浓度效应关系。细胞周期观察发现,SGC-7901细胞G1期细胞数随着CSO浓度的增加而递增,而S/G2期细胞随着浓度的细胞递降。Caspase-3、Caspase-9随着CSO浓度的逐渐增高表达上调,Bcl-2表达下调。结论鳕鱼皮寡肽能抑制人胃癌细胞(SGC-7901)增殖,诱导凋亡,抑癌机制可能与Caspase-3、Caspase-9上调和Bcl-2下调相关。Objective To explore the effect of cod skin oligopeptide（CSO） on the proliferation in human gastric carcinoma SGC-790l cells. Methods After treatment of SGC-7901 cells with different concentrations of CSO, the cells were observed under the microscope and the viability of cells was detected by CCK-8 assay. Cell apoptosis and cell cycle were determined by Western blotting and flow cytometry. Results CSO significantly suppressed the proliferation of SGC- 7901 cells in a time-and dose-dependent manner（P 〈 0.05）. The cultured SGC-7901 cells were treated by CSO for 24 hours and 48 hours and 72 hours with the IC50of 156.90 g/L and 102. 10 g/L and 73. 13 g/L. A large number of nuclear fragmentation and apoptotic bodies were observed under fluorescence microscope after treatment by DAPI. After treatment with CSO for 24 hours, SGC-7901 cells showed increased at G~ stage and cell cycle arrested at S/G2 stage, and the cell apoptosis rate increased with the CSO. With the increase of CSO concentration, the expression of Caspase-3 and Caspase-9 was up-regulated, and the expression of Bcl-2 was down-regulated. Conclusion CSO can promote the apoptosis and inhibit the proliferation of SGC-7901 cells. Anti-cancer mechanism may be related with Caspase-3 and Caspase-9 up- regulating,and Bcl-2 was down-regulating.

关 键 词：鳕鱼皮寡肽 胃癌 SGC-7901 Caspase-3 CASPASE-9 Bcl-2 免疫印迹法 流式细胞术 人 

分 类 号：R735.2[医药卫生—肿瘤]