三类生物因子对涡虫生殖和再生的影响  

Influence of three kinds of biological factors on the reproduction and regeneration in planarians

作　　者：董自梅[1] 董艳萍 陈广文[1] 刘德增[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《解剖学报》2017年第6期732-739,共8页Acta Anatomica Sinica

基　　金：国家自然科学基金(31570376;31471965;31170357;U1604173);河南省基础与前沿技术研究基金(162300410148)

摘　　要：目的以国内广布种日本三角涡虫(Dugesia japonica)为受试动物,探讨三类生物因子对涡虫生殖和再生的影响。方法选择与生殖相关的激素(雌二醇、甲基睾丸素),生物胺(5-羟色胺、多巴胺)和D-氨基酸(D-色氨酸、D-精氨酸、D-苯丙氨酸)对日本三角涡虫进行急性毒性实验,用SPSS 17.0统计学软件计算出适宜的实验浓度,对整体及再生涡虫进行培养,观察并记录实验现象。结果雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸处理组分别引起20%、40%、20%和20%涡虫卵巢及交配器官的发育;而多巴胺则引起40%~50%涡虫生殖腺退化。另外,5-羟色胺处理组涡虫头部再生速度加快,而多巴胺处理组则头部再生速度减慢。结论雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸能够诱导涡虫生殖腺的发育,并且5-羟色胺促进涡虫头部再生;多巴胺则引起涡虫生殖腺退化并对涡虫再生有抑制作用。Objective To investigate the effects of the three reproductive-relative factors on gonads and regeneration in planarians. Methods In this study, the planarians Dugesia japonica were cultured with three reproductive- relative factors： the hormones [ estradiol （ E2 ）, methyltestosteronum （MT） ~, biogenic amine [5-hydroxy-tryptamine （5-HT）, dopamine （DA） ] and D-amino acids （D-tryptophan, D-arginine, D-phenylalanine）. The cultured concentration was calculated by SPSS17.0 after the acute toxicity experiment, and the experimental phenomena were recorded. Results E2, 5-HT, D-tryptophan and D-phenylalanine improved the gonads development, which proportions were 20%, 40%, 20% , and 20% respectively, but DA made the gonads atrophy in 40% or 50% planarians. Besides, 5-HT increased speed of regeneration, while DA inhibited it during planarians regeneration. Conclusion E2, 5-HT, D-tryptophan and D- phenylalanine improved the gonads development, and 5-HT increased speed of regeneration; DA inhibited the gonads and regeneration.

关 键 词：性化 再生 激素 生物胺 D-氨基酸 急性毒性实验 日本三角涡虫 

分 类 号：Q954.2[生物学—动物学]