没食子多酚类化合物的鉴定及其在UPLC-MS/MS中的裂解规律研究  

Identification and fragmentation regularity of polyphenol compounds from Galla Quercina by UPLC-MS/MS

作　　者：马尚智 秦惠玉 龙飞[2] 于玮[1] 陈海君[1] 欧阳辉[3] 陈文[1] 韩博[1] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]石河子大学化学化工学院,新疆石河子832002 [3]石河子市人民医院,新疆石河子832003

出　　处：《中草药》2017年第22期4632-4638,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81560699);兵团科技攻关与成果转化计划项目(2015AD002);广东省科技资助项目(2014A020209026)

摘　　要：目的采用超高效液相色谱串联质谱技术(UPLC-MS/MS)分析没食子中多酚类化合物的裂解规律并鉴定其结构。方法采用Waters Xevo TQD液质联用仪,0.2%甲酸水溶液-乙腈为流动相,梯度洗脱,ESI负离子模式下采集数据,并通过Mass Lynx软件分析。结果鉴定出没食子中没食子酸鞣质、鞣花酸鞣质、酚酸类15个多酚类化合物,并发现没食子酸鞣质通过失去没食子酰基、C_2H_2O、CH_2O、CO_2和OH发生裂解,且各没食子酸鞣质间存在丢失没食子酰基(m/z 152)的规律。结论得到的质谱裂解规律可用于鉴定没食子中的多酚类化合物,也为研究富含多酚类成分的植物药的化学组成提供参考。Objective To explore the fragmentation regularity and identify the polyphenol components in Galla Quercina. Methods Waters Xevo TQD liquid chromatography/mass spectrometer ESI negative mode was used to analysis, mobile phase was 0.02% formic acid-water and acetonitrile for gradient eluent. Data were analyzed by Mass Lynx software. Results Three categories and 15 compounds in Galla Quercina were identified and divided into gallotannins, ellagitannins, and phenolic acids. Gallotannins was cleaved by loss of gallic acyl, C2 H2O, CH2O, CO2, and OH, and the gallotannins had the regular loss of gallic acid units（152）. Conclusion The results of fragmentation mechanism can be used to detect polyphenol compounds in Galla Quercina and provide a useful reference for the study on the chemical constituents of medicinal plants rich in polyphenols.

关 键 词：没食子 多酚 超高效液相色谱串联质谱技术 没食子酸鞣质 鞣花酸鞣质 酚酸类 

分 类 号：R284.1[医药卫生—中药学]