超高效液相色谱-串联质谱法测定猪肉中氯霉素残留  

Residue determination of chloramphenicol in pork by ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：赵世瑾 姚凯[1] 李娜 张欣达[1] 赵鹏跃 王翠翠[1] 王丹[1] 郭永泽 李存[1] 

机构地区：[1]天津农学院,天津300384 [2]天津市农业质量标准与检测技术研究所,天津300381

出　　处：《黑龙江畜牧兽医》2017年第12期249-252,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31372482);天津市大学生创新创业训练计划项目(201510061043)

摘　　要：为了采用超高效液相色谱-串联质谱(UPLC-MS/MS)法测定猪肉中氯霉素(CAP)的残留,试验采用水提取均质后的猪肉样品中的CAP,提取液用C18固相萃取柱(SPE)净化后,采用UPLCMS/MS电喷雾电离(ESI),负离子,多反应监测(MRM)模式检测,基质添加外标法定量。结果表明:CAP检出限为0.04 ng/g,定量限为0.15 ng/g。在添加浓度为0.2~2.0 ng/g范围内,CAP的回收率为51.2%~63.1%,日内相对标准偏差和日间相对标准偏差分别为3.1%~9.8%、6.1%~9.5%。To determine residue of chloramphenieol （CAP） in pork using ultra - high performance liquid chromatography - tandem mass spec- trometry（ UPLC -MS/MS）, CAP- contained pork was homogenized in water in this experiment and extracted liquid was purified by C is solid phase extraction cartridges. CAP determination was achieved using UPLC - MS/MS with the mode of electrospray ionization （ ESI）, negative ion and multi -reaction monitoring（MRM）. External standard method was adopted in matrix for CAP quantification. The results showed that limit of detection was 0.04 ng/g,and limit of quantification （LOQ） was 0.15 ng/g. The recovery of CAP was in the range from 51.2% to 63.1% under the concentration from 0.2 ng/g to 2.0 ng/g. Intraday and interday relative standard deviations ranged from 3.1% to 9.8% and from 6.1% to 9.5% ,respectively.

关 键 词：超高效液相色谱-串联质谱(UPLC—MS/MS) 猪肉 氯霉素(CAP) 残留 多反应监测 (MRM) 

分 类 号：S859.84[农业科学—临床兽医学]