荧光定量RT-PCR检测白血病多药耐药基因及其临床意义  

Quantifying MDR1 gene expression of leukemia using fluorescence -quantitation reverse transcription-polymerase chain reaction (RT-PCR) and its clinical meaning

作　　者：穆会君[1] 谢平[1] 

机构地区：[1]无锡市临床医学实验研究所,南京医科大学附属无锡第一医院中心实验室,江苏无锡214002

出　　处：《中国实验诊断学》2002年第4期214-215,共2页Chinese Journal of Laboratory Diagnosis

基　　金：江苏省 3 3 3工程资助项目 (编号 :CS2 0 0 13 0 0 1)

摘　　要：目的　在Light-CyclePCR仪上对多药耐药基因 (MDR1)定量的技术方法和临床应用进行探讨。方法　应用荧光定量逆转录 -多聚酶链反应 (RT -PCR)检测了 30例急性白血病患者和 8例正常人外周血MDR1基因的表达。结果　该实验方法标准曲线线性良好 (r=1.0 ) ,耐药组MDR1基因拷贝数与非耐药组有显著性差异 (P <0 .0 1) ,正常人亦有低水平MDR1基因的表达。结论　荧光逆转录 -多聚酶链反应 (RT -PCR)是一种可靠的定量方法 ,能快速、特异地检测临床肿瘤标本中MDR1,为临床医生用药、预防MDR发生、考核逆转MDR效果等方面提供了有价值的量化指标。Objective To study the method of quantifying MDR1 gene expression using the LightCycle PCR and its clinical application. Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results The standard curve of this method is good (The linear correlation coefficient is 1.00) . There is significant difference between the drug resistance group and the drug non-resistance group (P<0.01). Healthy persons as well contain low level MDR1 gene expression. Conclusion Our results indicate that fluorescence reverse transcription-polymerase chain reaction (RT-PCR) is a rapid , special and reliable method for quantifying MDR1 gene expression in clinical tumor samples. It provides a worthy parameter for clinical doctor using the medicine, preventing the MDR occurrence, monitoring conversion of the MDR gene .

关 键 词：荧光定量RT-PCR 白血病 多药耐药基因 临床意义 

分 类 号：R733.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]