DHA干预对GDM小鼠胎盘脂肪酸转运蛋白基因表达的影响  

The effect of docosahexaenoic acid intervention on the gene expression of placental fatty acid transporters in GDM mice

作　　者：马艳玲[1] 何修界[1] 胡传来[1] 赵奇红[1] 孙金华[1] 丁靓[1] 

机构地区：[1]安徽医科大学公共卫生学院营养与食品卫生学系,合肥230032

出　　处：《安徽医科大学学报》2017年第12期1764-1768,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81373011)

摘　　要：目的探讨二十二碳六烯酸(DHA)干预对妊娠期糖尿病(GDM)小鼠胎盘脂肪酸转运蛋白基因表达的影响。方法选取36只小鼠,其中12只作为空白对照(CN),24只腹腔注射链脲霉素建立GDM模型;GDM小鼠随机分为两组:DHA补充组(GDM+DHA)以及对照组(GDM)。于第10天~18天,给予GDM+DHA组灌胃DHA 500 mg/kg,其他两组灌胃等量溶剂,记录各时期3组血糖及体重水平,第18天收集孕鼠血清及胎盘。羟胺法和比色法分别测定母鼠血清超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-px)活力水平,RT-PCR法测定胎盘脂肪酸转运蛋白基因表达水平。结果 (1)重复测量资料的方差分析结果显示:不同时间段的孕鼠体重、血糖差异有统计学意义(P<0.05);不同组别孕鼠血糖差异有统计学意义(P<0.05)。(2)三组小鼠体内SOD活性、GSH-px活力水平差异有统计学意义(P<0.05);与CN组比,GDM组小鼠体内SOD和GSH-px活力水平显著降低(P<0.05),DHA干预后,小鼠体内SOD活力水平显著升高(P<0.05)。(3)与CN组比,GDM下调了过氧化物酶体增殖激活受体γ(PPARγ)mRNA表达,上调脂肪酸结合蛋白(FABP-pm)、脂肪酸转运蛋白-6(FATP-6 mRNA)表达,DHA干预后,下调了FABP-pm、FATP-6 mRNA的表达。结论DHA干预可增加GDM小鼠体内SOD活力,下调胎盘FABPpm、FATP-6 mRNA的表达。Objective To explore the effect of docosahexaenoic acid（ DHA） intervention on the gene expression of placental fatty acid transporters in gestational diabetes mellitus（ GDM） mice. Methods 12 mice were used as blank controls,while 24 mice were injected intraperitoneally with streptozotocin to establish GDM model. GDM mice were separated and randomized to DHA supplementary group and control group. The mice of DHA supplementary group were given 500 mg/kg of DHA via oral gavage between the 10 th day and the 18 th day. The others received solution without DHA at the same dose via oral gavage. The weight and glucose of all mice were collected at each time point. At the 18 th day,placentas and blood serum were collected. The level of superoxide dismutase（SOD） and glutathione peroxidase（GSH-px） were examined by hydroxylamine and colorimetry respectively. The gene expression of placental fatty acid transporters were examined by RT-PCR. Results（1） The repeated measures ANOVA showed that the body weight and serum glucose of pregnant mice were statistically significant difference at different time（ P〈0. 05）,and the serum glucose in different group was also different（ P〈0. 05）.（2） The level of SOD and GSH-px in different group were statistically significant different（ P〈0. 05）. Compared with CN group,the level of SOD and GSH-px were lower in GDM（ P〈0. 05）. After received DHA supplement,the level of SOD was significantly higher in GDM mice（ P〈0. 05）.（3） Compared with CN group,GDM down-regulated the mRNA expression of peroxisome proliferators-activated receptor gamma（ PPARγ）,while the mRNA expression of plasma membrane fatty acid binding proteins（ FABP-pm） and fatty acid-transport protein 6（ FATP-6） mRNA were up-regulated. After received DHA supplement,the mRNA expression of FABP-pm and FATP-6 were down-regulated.Conclusion DHA supplement can increase the level of SOD and down-regulate the mRNA expression of FABP-pm and FATP-6 in GDM mice.

关 键 词：妊娠期糖尿病 脂肪酸转运蛋白 DHA PPARΓ 

分 类 号：R151.2[医药卫生—营养与食品卫生学]